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Plant and Cell Physiology Advance Access published online on April 27, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm051
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen:

Pei-Luen Jiang1, Co-Shing Wang1, Chia-Mei Hsu2, Guang-Yuh Jauh2,* and Jason T.C. Tzen1,3,*

1 Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan
2 Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan
3 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan

*To whom correspondence should be addressed: Guang-Yuh Jauh. Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan, Phone: 886- 2-27899590, E mail address: jauh{at}gate.sinica.edu.tw, Jason T.C. Tzen, Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan, 40227, Phone: 886-4-22840328, Fax: 886-4-22853527, E mail address: TCTZEN{at}dragon.nchu.edu.tw


  Abstract

Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. Integrity of pollen oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies and massively accumulated in late stages of pollen maturation. According to mass spectrometric analyses, this oil-body protein possessed a trypic fragment of 13 residues matching to that of a theoretical rice oleosin. A complete cDNA fragment encoding this putative oleosin was obtained by PCR cloning with primers derived from its known 13-residue sequence. Sequence analysis as well as immunological non-cross recognition suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. In pollen cells observed under electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route in contrast with the glyoxysomal mobilization of seed oil bodies after germination.

Keywords: germination - lily (Lilium longiflorum Thunb - pollen - oil bodies - oleosin


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P.-L. Jiang, G.-Y. Jauh, C.-S. Wang, and J. T.C. Tzen
A Unique Caleosin in Oil Bodies of Lily Pollen
Plant Cell Physiol., September 1, 2008; 49(9): 1390 - 1395.
[Abstract] [Full Text] [PDF]


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