Skip Navigation



Plant and Cell Physiology Advance Access published online on April 22, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm047
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
48/6/875    most recent
pcm047v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ventriglia, T.
Right arrow Articles by Romero, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ventriglia, T.
Right arrow Articles by Romero, J. M.
Agricola
Right arrow Articles by Ventriglia, T.
Right arrow Articles by Romero, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regulatory properties of potato-Arabidopsis hybrid ADP-Glucose Pyrophosphorylase

Tiziana Ventriglia1, Miguel A. Ballicora2,3, Pedro Crevillén1,4, Jack Preiss2 and José M. Romero1,*

1 Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla-C.S.I.C., c/ Americo Vespucio N° 49, 41092-Sevilla, Spain
2 Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824.
3 Present address: Dept. of Chemistry, Loyola University Chicago. 405 Flanner Hall, 1068 W. Sheridan Rd. Chicago, IL 60626.
4 Present Address: Department of Cell and Development Biology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, U.K.

*Corresponding author: José M. Romero. Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla-C.S.I.C., c/ Americo Vespucio N° 49, 41092-Sevilla, Spain. Telf: +34-954489526, FAX: + 34-954460065, E-mail: jmromero{at}ibvf.csic.es


  Abstract

In higher plants, ADP-glucose pyrophosphorylase (ADPGlc-PPase) is a heterotetrameric enzyme comprised by two small and two large subunits. Potato/Arabidopsis hybrid ADPGlc-PPases were generated and their regulatory properties analyzed. We show that ADPGlc-PPase subunits from two different species can interact rendering active enzymes with new regulatory properties. Depending on subunit combinations, hybrid heterotetramers showed responses to the allosteric effectors (3-PGA and Pi) in the µM or mM range. While hybrid potato small subunit (PSS) and the Arabidopsis large subunit APL1 showed an extremely sensitive response to 3-PGA and Pi, hybrid PSS/Arabidopsis APL2 was very insensitive to them. Intermediate responses were determined for other subunit combinations.

Keywords: ADP-glucose pyrophosphorylase - allosteric regulation - Arabidopsis thaliana - heterotetrameric enzyme - hybrid-enzyme - potato


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
T. Ventriglia, M. L. Kuhn, M. T. Ruiz, M. Ribeiro-Pedro, F. Valverde, M. A. Ballicora, J. Preiss, and J. M. Romero
Two Arabidopsis ADP-Glucose Pyrophosphorylase Large Subunits (APL1 and APL2) Are Catalytic
Plant Physiology, September 1, 2008; 148(1): 65 - 76.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.