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Plant and Cell Physiology Advance Access published online on April 22, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm045
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Excessive expression of the plant kinesin TBK5 converts cortical and perinuclear microtubules into a radial array emanating from a single focus

Yuhei Goto* and Tetsuhiro Asada

Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan

Corresponding author: Tetsuhiro Asada. Department of Biological Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan. Tel: Tel: 06-6850-6776, FAX: Fax: 06-6850-5808, E-mail: tasada{at}bio.sci.osaka-u.ac.jp


  Abstract

TBK5 is a plant-specific kinesin constantly expressed in tobacco BY-2 cells. An analysis of the distribution of green fluorescent protein-tagged TBK5 (GFP-TBK5) transiently expressed in BY-2 protoplasts revealed that TBK5 could associate with microtubules in vivo. GFP-TBK5 often assembled to form a single particle when accumulated in cells. The particle was located in close proximity to the nucleus, and its formation was accompanied by the development of a radial array of microtubules emanating from it and the loss of cortical microtubules. Microtubule depolymerization inhibited particle formation and stimulated the formation of dispersed aggregates of GFP-TBK5. Through expression of different TBK5 mutants as GFP fusions, the motor domain, two separated coiled-coil domains, and the C-terminal domain of TBK5 were identified as the domains playing essential roles in particle formation. Mutants with putatively non-motile motor domains or lacking the C-terminal domain were localized to cortical and perinuclear microtubules, whereas lacking either of the coiled-coil domains were preferentially distributed around the nucleus and along perinuclar microtubules. Further, the deletion of one of the coiled-coil domains or the C-terminal domain was sufficient to inhibit the propyzamide-induced formation of dispersed aggregates, whereas the mutation in the motor domain was not. These results led us to propose a model in which the particle is formed through the microtubule-based movement of GFP-TBK5 toward the nucleus and subsequent microtubule-independent aggregation based on coiled-coil interactions. The dramatic microtubule rearrangement would be explained if GFP-TBK5 relocated and gathered newly formed microtubules and/or microtubule-nucleating units.

Keywords: Microtubule - Kinesin - Microtubule-organizing center - Cortical microtubules - Tobacco BY-2 cell

*Present address: Research and Development Division, Janssen Pharmaceutical Co. Ltd., Shinagawa, Tokyo 141-8633, Japan


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