Plant and Cell Physiology Advance Access published online on March 26, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm039
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Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cells
1Department of Biology, Faculty of Science, Kyushu University, Ropponmatsu, Fukuoka 810-8560, Japan
2Henan Key Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, Kaifeng 475001, People’s Republic of China
*For correspondence; (Fax +81 92 726 4758; e-mail kenrcb{at}mbox.nc.kyushu-u.ac.jp
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Abstract |
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Recent evidence suggests that nitric oxide (NO) acts as an intermediate of abscisic acid (ABA) signal transduction for stomatal closure. However, NO’s effect on stomatal opening is poorly understood even though both opening and closing activities determine stomatal aperture. Here we show that NO inhibits stomatal opening specific to blue light, thereby stimulating stomatal closure. NO inhibited blue light-specific stomatal opening but not red light-induced opening. NO inhibited both blue light-induced H+ pumping and H+-ATPase phosphorylation. NO scavenger 2-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) restored all these inhibitions. ABA and hydrogen peroxide (H2O2) inhibited all of these blue light-specific responses in a manner similar to NO. c-PTIO partially restored the ABA-induced inhibition of all of these opening responses but did not restore inhibition of the responses by H2O2. ABA, H2O2, and NO had slight inhibitory effects on the phosphorylation of phototropins, which are blue light receptors in guard cells. NO inhibited neither fusicoccin-induced H+ pumping in guard cells nor H+ transport by H+-ATPase in the isolated membranes. From these results, we conclude that both NO and H2O2 inhibit blue light-induced activation of H+-ATPase by inhibiting the component(s) between phototropins and H+-ATPase in guard cells and stimulate stomatal closure by ABA.
Keywords: abscisic acid - blue light - H+ pumping - nitric oxide - stomatal opening
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