Skip Navigation


Plant and Cell Physiology Advance Access first published online on February 27, 2007
This version published online on March 5, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm029
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
48/4/648    most recent
pcm029v2
pcm029v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mimida, N.
Right arrow Articles by Ichikawa, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mimida, N.
Right arrow Articles by Ichikawa, H.
Agricola
Right arrow Articles by Mimida, N.
Right arrow Articles by Ichikawa, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the Japanese Society of Plant Physiologists. All rights reserved.

Two Alternatively Spliced Transcripts Generated from OsMUS81, a Rice Homologue of Yeast MUS81, Are Upregulated by DNA-Damaging Treatments

Naozumi Mimida1,3, Hiroko Kitamoto1,4, Keishi Osakabe1, Marina Nakashima1, Yuji Ito1,5, Wolf-Dietrich Heyer2, Seiichi Toki1 and Hiroaki Ichikawa1,*

1 National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba 305-8602, Japan
2 Section of Microbiology and Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, California 95616-8665, USA

* Corresponding author: E-mail, hichkw{at}affrc.go.jp Fax, +81-29-838-7073.


  Abstract

OsMUS81, a rice homologue of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81{alpha} and OsMUS81ß. OsMus81{alpha} contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while OsMus81ß isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and gamma radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest OsMus81 functions in maintaining genome integrity through homologous recombination.

Keywords: Alternative splicing - DNA repair - Homologous recombination - MUS81 - Rice - XPF endonuclease superfamily

3 Present address: Department of Apple Research, National Institute of Fruit Tree Science, 92 Nabe-yashiki, Shimo-kuriyagawa, Morioka 020-0123, Japan

4 Present address: Environmental Biofunction Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604 Japan

5 Present address: National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira-ku, Sapporo, Hokkaido 062-8555, Japan


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Matulova, V. Marini, R. C. Burgess, A. Sisakova, Y. Kwon, R. Rothstein, P. Sung, and L. Krejci
Cooperativity of Mus81{middle dot}Mms4 with Rad54 in the Resolution of Recombination and Replication Intermediates
J. Biol. Chem., March 20, 2009; 284(12): 7733 - 7745.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. M. Mazina and A. V. Mazin
Human Rad54 protein stimulates human Mus81-Eme1 endonuclease
PNAS, November 25, 2008; 105(47): 18249 - 18254.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.