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Plant and Cell Physiology Advance Access published online on February 9, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm022
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© The Author 2007. Published by Oxford University Press on behalf of the Japanese Society of Plant Physiologists. All rights reserved.
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Over-expression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism

Naoya Hirose, Nobue Makita, Mikiko Kojima, Tomoe Kamada-Nobusada and Hitoshi Sakakibara*

RIKEN Plant Science Center, 1-7-22, Suehiro, Tsurumi, Yokohama 230-0045, Japan

*Corresponding author; e-mail sakaki{at}riken.jp.


  Abstract

Genome-wide analyses of rice (Oryza sativa L.) cytokinin (CK)-responsive genes using the Affymetrix GeneChip® rice genome array were conducted to define the spectrum of genes subject to regulation by CK in monocotyledonous plants. Application of trans-zeatin modulated the expression of a wide variety of genes including those involved in hormone signaling and metabolism, transcriptional regulation, macronutrient transport, and protein synthesis. To further understand the function of CK in rice plants, we examined the effects of in planta manipulation of a putative CK signaling factor on morphology, CK metabolism, and expression of CK-responsive genes. Over-expression of the CK-inducible type-A response regulator OsRR6 abolished shoot regeneration, suggesting that OsRR6 acts as a negative regulator of CK signaling. Transgenic lines over-expressing OsRR6 (OsRR6-ox) had dwarf phenotypes with poorly developed root systems and panicles. Increased content of trans-zeatin type CKs in OsRR6-ox lines indicates that homeostatic control of CK levels is regulated by OsRR6 signaling. Expression of genes encoding CK oxidase/dehydrogenase decreased in OsRR6-ox plants, possibly accounting for elevated CK levels in transgenic lines. Expression of a number of stress response genes was also altered in OsRR6-ox plants.

Keywords: cytokinin - gene chip - microarray - Oryza sativa - response regulator

Nucleotide sequence data from this article have been deposited in the DDBJ/EMBL/GenBank database under accession numbers AB249653 [GenBank] (OsRR6), AB249654 [GenBank] (OsRR5), AB249655 [GenBank] (OsRR3), AB249656 [GenBank] (OsRR11), AB249657 [GenBank] (OsRR7), AB249658 [GenBank] (OsRR4), AB249659 [GenBank] (OsRR9), AB249660 [GenBank] (OsRR10), AB249661 [GenBank] (OsRR1), AB249662 [GenBank] (OsRR2), AB249663 [GenBank] (OsRR8), AB249664 [GenBank] (OsRR12), and AB249665 [GenBank] (OsRR13). Microarray data have been deposited in The National Center for Biotechnology Information Gene Expression Omnibus (NCBI GEO) database under accession number GSE6737 [NCBI GEO] .


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