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Plant and Cell Physiology Advance Access published online on February 8, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm020
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Glucose-6-phosphate Dehydrogenase Plays a Pivotal Role in Nitric Oxide-Involved Defense Against Oxidative Stress Under Salt Stress in Red Kidney Bean Roots

Yinggao Liu, Ruru Wu, Qi Wan, Gengqiang Xie and Yurong Bi*

Key Laboratory of Arid and Grassland Agroecology (Ministry of Education), School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China

Corresponding author. Yurong Bi Address. Key Laboratory of Arid and Grassland Agroecology (Ministry of Education), School of Life Sciences, Lanzhou University, Lanzhou 730000, People's Republic of China Tel. 86-931-8912566 Fax. 86-931-8912561 e-mail. yrbi{at}lzu.edu.cn, biyurong{at}hotmail.com


  Abstract

The pivotal role of glucose-6-phosphate dehydrogenase (G-6-PDH)-mediated nitric oxide (NO) production in the tolerance to oxidative stress induced by 100 mM NaCl in red kidney bean (Phaseolus vulgaris) roots was investigated. The results show that the G-6-PDH activity was enhanced rapidly in the presence of NaCl and reached a maximum at 100 mM. Western-blot analysis indicated that the increase of G-6-PDH activity in the red kidney bean roots under 100 mM NaCl was mainly due to the increased content of the G-6-PDH protein. NO production and nitrate reductase (NR) activity were also induced by 100 mM NaCl. The NO production was reduced by NaN3 (NR inhibitor), but not affected by N{omega}-nitro-L-arginine (L-NNA) (NOS inhibitor). Application of 2.5 mM Na3PO4, an inhibitor of G-6-PDH, blocked the increase of G-6-PDH and NR activity, as well as NO production in red kidney bean roots under 100 mM NaCl. The activities of antioxidant enzymes in red kidney bean roots increased in the presence of 100 mM NaCl or sodium nitroprusside (SNP), a NO donor. The increased activities of all antioxidant enzymes tested at 100mM NaCl were completely inhibited by 2.5 mM Na3PO4. Based on these results, we conclude that G-6-PDH plays a pivotal role for NR-dependent NO production, and in establishing tolerance of red kidney bean roots to salt stress.

Keywords: Glucose-6-phosphate dehydrogenase - nitrate reductase - nitric oxide - red kidney bean - salt stress


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