Skip Navigation



Plant and Cell Physiology Advance Access published online on February 5, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm017
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
48/3/459    most recent
pcm017v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kodama, Y.
Right arrow Articles by Kato, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kodama, Y.
Right arrow Articles by Kato, K.
Agricola
Right arrow Articles by Kodama, Y.
Right arrow Articles by Kato, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Mapping and characterization of DNase I hypersensitive sites in Arabidopsis chromatin

Yuichi Kodama1, Shingo Nagaya1, Atsuhiko Shinmyo and Ko Kato2

Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan

2Corresponding author: kou{at}bs.naist.jp; Fax: +81-743-72-5469


  Abstract

Recent genome-wide analyses of yeast and human chromatin revealed the widespread prevalence of DNase I hypersensitive sites (DNase I HSs) at gene regulatory regions with possible roles in eukaryotic gene regulation. The presence of DNase I HSs in plants has been described for only a few genes, and we analyzed the chromatin structure of an 80-kb genomic region containing variably-expressed 30 genes by DNase I sensitivity assay at 500-bp resolution in Arabidopsis. Distinct DNase I HSs were found at the 5’ and/or 3’ ends of most genes irrespective of their expression levels. Further analysis of well-characterized genes showed that the DNase I HSs occurred near cis-regulatory elements in the promoters of these genes. Upon transcriptional activation of a heat-inducible gene, the DNase I HS was extended into the vicinity of a cis-element and adjacent TATA element in the promoter. Concomitant with this change in DNase I HS, histones were acetylated, removed from the promoter, and a transcription activator bound to this cis-element. These results suggest that the DNase I HSs participate in the transcriptional regulation of Arabidopsis genes by enhancing the access of chromatin remodeling factors and/or transcription factors to their target sites as seen in yeast and human chromatin.

Keywords: Arabidopsis thaliana - chromatin structure - DNase I hypersensitive site – gene expression - histone acetylation - transcription regulation

1These authors contributed equally to this work


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.