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Plant and Cell Physiology Advance Access first published online on February 6, 2007
This version published online on February 17, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm015
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Large Scale Analysis of Chlorophyll Fluorescence Kinetics in Synechocystis sp. PCC 6803: Identification of the Factors Involved in the Modulation of Photosystem Stoichiometry

Hiroshi Ozaki1, Masahiko Ikeuchi2, Teruo Ogawa3, Hideya Fukuzawa4 and Kintake Sonoike1,*

1 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba 277-8562, Japan
2 Department of Life Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan
3 Bioscience Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan
4 Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake-cho, Kyoto 606-8502, Japan

* Author for correspondence (e-mail: sonoike{at}k.u-tokyo.ac.jp)


  Abstract

Since chlorophyll fluorescence reflects the redox state of photosynthetic electron transport chain, monitoring of chlorophyll fluorescence was successfully applied for the screening of photosynthesis-related genes. Here we report that the mutants having defect in the regulation of photosystem stoichiometry could be identified through the simple comparison of the induction kinetics of chlorophyll fluorescence. We made a library containing 500 mutants in the cyanobacterium Synechocystis sp. PCC 6803 with transposon mediated gene disruption, and the mutants were served for the measurement of chlorophyll fluorescence kinetics for 45 seconds. We picked up two genes, pmgA and sll1961, which are involved in the modulation of photosystem stoichiometry. The disruptants of the two genes share the common characteristics in the fluorescence kinetics, and we searched for the mutants that showed such characteristics. Out of six mutants identified so far, five mutants showed different photosystem stoichiometry under high-light condition. Thus, the categorization based on the similarity of the fluorescence kinetics is an excellent way to identify the function of genes.

Keywords: Chlorophyll fluorescence - Cyanobacteria - Function of gene - Mutant library - Photosystem stoichiometry


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