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Plant and Cell Physiology Advance Access published online on January 23, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm012
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Roles for the N- and C-terminal domains of phytochrome B in interactions between phytochrome B and cryptochrome signaling cascades.

Takeshi Usami1, Tomonao Matsushita, Yoshito Oka2, Nobuyoshi Mochizuki and Akira Nagatani*

Department of Botany, Graduate School of Science, Kyoto University, Sakyo-Ku, Kyoto 606-8502, JAPAN

*Corresponding author: E-mail, nagatani{at}physiol.bot.kyoto-u.ac.jp; Fax, +81-75-753-4126.


  Abstract

Plants fine-tune light responses through interactions between photoreceptors. We have previously reported that the greening of Arabidopsis thaliana roots is regulated synergistically by phytochromes and cryptochromes. In the present study, we investigated the functions of the N-terminal and C-terminal domains of phytochrome B (phyB) in the interactions between phytochrome B and cryptochrome signaling cascades. Transgenic Arabidopsis expressing the phyB N-terminal domain fused to GFP, GUS, and NLS showed intense root greening under blue light, indicating that the C-terminal domain was dispensable for the synergistic interaction in the induction of root greening. However, root greening under red light was substantially reduced in the absence of the C-terminal domain. This effect was opposite to the previous observation that removal of the C-terminal domain enhanced the signaling activity of phyB in the inhibition of hypocotyl elongation. In addition, we found that over-expression of the isolated C-terminal domain of phyB enhanced the blue light response not only for root greening but also for the inhibition of hypocotyl elongation. Analysis of this activity on various photoreceptor mutant backgrounds demonstrated that the isolated C-terminal domain enhanced cryptochrome signaling. In summary, these results demonstrate that different domains of phyB can play various roles which are dependent on light conditions as well as on the specific physiological response.

Keywords: cryptochrome - hypocotyl elongation - photomorphogenesis - photoreceptor interaction - phytochrome - root greening

1 Present address: Department of Plant Developmental Genetics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, JAPAN

2 Present address: Department of Plant Physiology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, JAPAN


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A. Castillon, H. Shen, and E. Huq
Blue Light Induces Degradation of the Negative Regulator Phytochrome Interacting Factor 1 to Promote Photomorphogenic Development of Arabidopsis Seedlings
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[Abstract] [Full Text] [PDF]


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