Skip Navigation



Plant and Cell Physiology Advance Access published online on January 18, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm009
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
48/3/405    most recent
pcm009v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lee, E.-J.
Right arrow Articles by Koizumi, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, E.-J.
Right arrow Articles by Koizumi, N.
Agricola
Right arrow Articles by Lee, E.-J.
Right arrow Articles by Koizumi, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Glycosyl hydrolases of cell wall are induced by sugar starvation in Arabidopsis

Eun-Jeong Lee1, Yasuhiro Matsumura1, Kouichi Soga2, Takayuki Hoson2 and Nozomu Koizumi1

1 Nara Institute of Science and Technology, Graduate School of Biological Science, Nara 630-0192, Japan
2 Department of Biological Sciences, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan

Corresponding author: Nozomu Koizumi, Nara Institute of Science and Technology, Nara 630-0192, Japan; tel +81-743-72-5652; fax +81-743-72-5659;e-mail nkoizumi{at}gtc.naist.jp


  Abstract

Three Arabidopsis genes encoding a putative ß-galactosidase (At5g56870), ß-xylosidase (At5g49360) and ß-glucosidase (At3g60140) are induced by sugar starvation. The deduced proteins belong to the family 35, 3 and 1 glycosyl hydrolases, respectively. They are predicted to be secretory proteins that play roles in modification of cell wall polysaccharides based on amino acid similarity. The ß-galactosidase encoded by At5g56870 was identified as a secretory protein in culture medium of suspension cells by mass spectrometry analysis. This protein was specifically detected under sugar-starved condition with a specific antibody. Induction of these genes was repressed in suspension cells grown with galactose, xylose and glucose as well as with sucrose. In planta, expression of the genes and protein accumulation were detected when photosynthesis was inhibited. Glycosyl hydrolase activity against galactan also increased during sugar starvation. The amount of monosaccharide in pectin and hemicellulose in detached leaves decreased in response to sugar starvation. These findings suggest that the cell wall may function as a storage reserve of carbon in addition to providing physical support of the plant body.

Keywords: Arabidopsis - cell wall - glycosyl hydrolases - sugar starvation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.