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Plant and Cell Physiology Advance Access published online on January 4, 2007
Plant and Cell Physiology, doi:10.1093/pcp/pcm001
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Microtubule- and actin filament-dependent motors are distributed on pollen tube mitochondria and contribute differently to the their movement

Silvia Romagnoli1, Giampiero Cai1, Claudia Faleri1, Etsuo Yokota2, Teruo Shimmen2 and Mauro Cresti1

1Dipartimento Scienze Ambientali "G. Sarfatti", Università di Siena, via Mattioli 4, 53100 Siena, Italy
2Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, Hyogo 678-12, Japan

Corresponding author Mauro Cresti, Dipartimento Scienze Ambientali, Università di Siena, via Mattioli 4, I-53100 Siena (Italy); e-mail: cai{at}unisi.it; fax: +39-0577-232860; phone: +39-0577-232891


  Abstract

The pollen tube exhibits cytoplasmic streaming of organelles, which is dependent on the actin-myosin system. Although microtubule-based motors have been also identified in the pollen tube, many uncertainties exist on their role in organelle transport. As part of our attempt to understand the role of microtubule-based movement in the pollen tube of tobacco, we investigated the cooperation between microtubules and actin filaments in the transport of mitochondria and Golgi vesicles, which are distributed differently in the growing pollen tube. The analysis was performed using in vitro motility assays in which organelles move along both microtubules and actin filaments. Results indicated that the movement of mitochondria and Golgi vesicles is slow and continuous along microtubules but fast and irregular along actin filaments. In addition, the presence of microtubules in the motility assays force organelles to use lower velocities. Actin- and tubulin-binding tests, immunoblotting, and immunogold labelling indicated that different organelles bind to identical myosins but associate with specific kinesins. We found that a 90-kD kinesin (previously known as 90-kD ATP-MAP) is associated with mitochondria but not with Golgi vesicles, whereas a 170-kD myosin is distributed on mitochondria and other organelle classes. In vitro and in vivo motility assays indicate that microtubules and kinesins decrease the speed of mitochondria thus contributing to their positioning in the pollen tube.

Keywords: kinesin - microtubule - mitochondria - myosin - organelle movement - pollen tube


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