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Plant and Cell Physiology Advance Access published online on December 5, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl051
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Bowman-Birk Proteinase Inhibitor Confers Heavy Metal and Multiple Drug Tolerance in Yeast

Nobukazu Shitan1, Ken-ichi Horiuchi2, Fumihiko Sato3 and Kazufumi Yazaki1,*

1Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, Japan
2Materials and Analyses Team, Core Technology Development Division, Technical Center, Nitta Corporation, 172, Ikezawacho, Yamatokoriyama, Nara, 639-1085, Japan
3Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan

*To whom correspondence should be addressed. Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, Japan, E-mail: yazaki{at}rish.kyoto-u.ac.jp, Tel.: +81-774-38-3617, Fax: +81-774-38-3623


  Abstract

Cultured Coptis japonica cells show tolerance to various toxic compounds. By yeast functional screening of cadmium (Cd) plate with its cDNA library, we isolated a gene encoding Bowman-Birk proteinase inhibitor (CjBBI). The yeast transformant of CjBBI showed multiple tolerance to various drugs adding to Cd, and revealed reduced Cd accumulation in cells. Preferential organs for Cjbbi expression were aerial parts of intact plants, and the subcellular localization of CjBBI was shown using its GFP fusion to be apoplast. Induction of Cjbbi expression by Cd treatment suggested that CjBBI was responsible for the tolerance to Cd observed in C. japonica cells.

Keywords: Alkaloid - Cadmium - Coptis japonica - Functional screening - Multiple drug tolerance - Proteinase inhibitor


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