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Plant and Cell Physiology Advance Access published online on November 10, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl033
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular paper

Proteome analysis of bell pepper (Capsicum annum L.) chromoplasts

Muhammad Asim Siddique 1, Jonas Grossmann 1, Wilhelm Gruissem 1, and Sacha Baginsky 1 *

1 Institute of Plant Sciences, ETH Zurich, Universitätstrasse 2, CH-8092 Zurich, Switzerland

* To whom correspondence should be addressed.
Sacha Baginsky, E-mail: sbaginsky{at}ethz.ch


  Abstract

We report a comprehensive proteome analysis of chromoplasts from bell pepper (Capsicum annum L.). The combination of a novel strategy for database-independent detection of proteins from MS/MS data with standard database searches allowed us to identify 151 proteins with high confidence. These include several well-known plastid proteins but also novel proteins that were not previously reported from other plastid proteome studies. The majority of the identified proteins are active in plastid carbohydrate and amino acid metabolism. Among the most abundant individual proteins are capsanthin/capsorubin synthase and fibrillin, which are involved in the synthesis and storage of carotenoids that accumulate to high levels in chromoplasts. The relative abundances of the identified chromoplast proteins differ remarkably compared to their abundances in other plastid types, suggesting a chromoplast-specific metabolic network. Our results provide an overview of the major metabolic pathways active in chromoplasts and extend existing knowledge about prevalent metabolic activities of different plastid types.

Keywords: Chromoplast; proteomics; mass spectrometry; metabolism; carotenoid biosynthesis.
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