Skip Navigation



Plant and Cell Physiology Advance Access published online on October 20, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl022
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
47/11/1572    most recent
pcl022v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shimomura, K.
Right arrow Articles by Kouchi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimomura, K.
Right arrow Articles by Kouchi, H.
Agricola
Right arrow Articles by Shimomura, K.
Right arrow Articles by Kouchi, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular paper

LjnsRING, a Novel RING Finger Protein, is Required for Symbiotic Interactions Between Mesorhizobium loti and Lotus japonicus

Kenshiro Shimomura 1, Mika Nomura 2, Shigeyuki Tajima 2, and Hiroshi Kouchi 3 *

1 Faculty of Agriculture, Kagawa University, Miki, Kita, Kagawa, 761-0795 Japan; Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan
2 Faculty of Agriculture, Kagawa University, Miki, Kita, Kagawa, 761-0795 Japan
3 Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan

* To whom correspondence should be addressed.
Hiroshi Kouchi, E-mail: kouchih{at}nias.affrc.go.jp


  Abstract

Nodule-specific (nodulin) genes are thought to play crucial roles during establishment of the nitrogen-fixing symbiosis between legume plants and Rhizobium bacteria. On the basis of a gene expression database for early stages of the nodulation process of Lotus japonicus, previously constructed by a cDNA macroarray analysis, we identified a novel nodulin gene, LjnsRING, which encodes a protein with a typical RING-H2 finger domain that is well-conserved in a number of plant E3 ubiquitin ligases. LjnsRING transcripts were almost exclusively expressed in nodules, and very low expression was detected in roots and shoots. RNA interference (RNAi) knockdown of LjnsRING by hairy root transformation caused impaired root growth together with abortion of nodule formation. Examination with lacZ-labelled Mesorhizobium loti indicated that infection thread formation in the RNAi transgenic hairy roots was significantly inhibited. Analysis using a chimeric gene of LjnsRING promoter and {beta}-glucuronidase (GUS) coding region demonstrated that LjnsRING transcription in nodules was restricted to the infected cells. These results suggest the requirement of LjnsRING in rhizobial infection and subsequent nodule formation process.

Keywords: Lotus japonicus; Mesorhizobium loti; nodulation; RNAi; Agrobacterium rhizogenes.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
E. Kiss, B. Olah, P. Kalo, M. Morales, A. B. Heckmann, A. Borbola, A. Lozsa, K. Kontar, P. Middleton, J. A. Downie, et al.
LIN, a Novel Type of U-Box/WD40 Protein, Controls Early Infection by Rhizobia in Legumes
Plant Physiology, November 1, 2009; 151(3): 1239 - 1249.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Den Herder, A. De Keyser, R. De Rycke, S. Rombauts, W. Van de Velde, M. R. Clemente, C. Verplancke, P. Mergaert, E. Kondorosi, M. Holsters, et al.
Seven in Absentia Proteins Affect Plant Growth and Nodulation in Medicago truncatula
Plant Physiology, September 1, 2008; 148(1): 369 - 382.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.