Skip Navigation



Plant and Cell Physiology Advance Access published online on October 20, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl021
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
47/11/1555    most recent
pcl021v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yoshida, K.
Right arrow Articles by Komae, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshida, K.
Right arrow Articles by Komae, K.
Agricola
Right arrow Articles by Yoshida, K.
Right arrow Articles by Komae, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular paper

Carbohydrate binding module of a rice endo-{beta}-1,4-glycanase, OsCel9A, expressed in auxin-induced lateral root primordia, is posttranslationally truncated

Kouki Yoshida 1 *, Nobuyuki Imaizumi 2, Satoshi Kaneko 3, Yasushi Kawagoe 4, Akemi Tagiri 4, Hiroshi Tanaka 4, Kazuhiko Nishitani 5, and Kozo Komae 6

1 Taisei Co., 344-1 Nase-cho, Totuka-ku, Yokohama 245-0051, Japan
2 Japan Turfgrass Inc., 3-6-3 Akanehama, Narashino-shi, Chiba 275-0024, Japan
3 National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan
4 National Institute for Agrobiological Science, 2-1-2 Kannnondai, Tsukuba-shi, Ibaraki 305-8518, Japan
5 Tohoku University, Sendai 980-8578, Japan
6 National Institute of Crop Science, 2-1-18 Kannondai, Tuskuba-shi, Ibaraki 305-8518, Japan

* To whom correspondence should be addressed.
Kouki Yoshida, E-mail: kouki.yoshida{at}sakura.taisei.co.jp


  Abstract

We report the cloning of a glycoside hydrolase family (GHF) 9 gene of rice (Oryza sativa L. cv. Sasanishiki), OsCel9A, corresponding to the auxin-induced 51 kDa endo-1,4-{beta}-glucanase (EGase). This enzyme reveals a broad substrate specificity with respect to sugar backbones (glucose and xylose) in {beta}-1,4-glycans of type II cell wall. OsCel9A encodes a 640 amino acid polypeptide and is an ortholog of TomCel8, a tomato EGase containing a carbohydrate binding module family (CBM) 2 sequence at its carboxyl (COOH) terminus. The expression of four rice EGase genes including OsCel9A showed different patterns of organ specificity and responses to auxin. OsCel9A was preferentially expressed during the initiation of lateral roots or subcultured root calli but was hardly expressed during auxin-induced coleoptile elongation or in seed calli in contrast to OsCel9D, a KORRIGAN (KOR) homolog. In situ localization of OsCel9A transcripts demonstrated that its expression was specifically upregulated in lateral root primordia (LRP). Northern blotting analysis showed the presence of a single product of OsCel9A. In contrast, both mass spectrometric analyses of peptide fragments from purified 51 kDa EGase proteins and immunogel blot analysis of EGase proteins in root extracts using two antibodies against internal peptide sequences of OsCel9A revealed that the entire CBM2 region was posttranslationally truncated from the 67 kDa nascent protein to generate 51 kDa EGase isoforms. Analyses of auxin concentration- and time course-dependence of accumulation of two EGase isoforms suggested that the translation and posttranslational CBM2-truncation of the OsCel9A gene may participate in lateral root development.

Keywords: Rice; Auxin; Glycoside hydrolase family 9; Lateral root formation; Processing; Carbohydrate binding module family 2.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
J. Takahashi, U. J. Rudsander, M. Hedenstrom, A. Banasiak, J. Harholt, N. Amelot, P. Immerzeel, P. Ryden, S. Endo, F. M. Ibatullin, et al.
KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems
Plant Cell Physiol., June 1, 2009; 50(6): 1099 - 1115.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Yoshida and K. Komae
A Rice Family 9 Glycoside Hydrolase Isozyme with Broad Substrate Specificity for Hemicelluloses in Type II Cell Walls
Plant Cell Physiol., November 1, 2006; 47(11): 1541 - 1554.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.