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Plant and Cell Physiology Advance Access published online on October 3, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl018
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Short Communication

Genetic transformation of Populus trichocarpa genotype Nisqually-1: A functional genomic tool for woody plants

Jingyuan Song 1, Shanfa Lu 2, Zenn-Zong Chen 3, Rodrigo Lourenco 2, and Vincent L. Chiang 2 *

1 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094, P.R. China; Forest Biotechnology Group, Department of Forestry and Environmental Resources, College of Natural Resources, North Carolina State University, Raleigh, NC 27695, USA
2 Forest Biotechnology Group, Department of Forestry and Environmental Resources, College of Natural Resources, North Carolina State University, Raleigh, NC 27695, USA
3 Division of Silviculture, Taiwan Forestry Research Institute, 53 Nan-Hai Road, Taipei 100, Taiwan

* To whom correspondence should be addressed.
Vincent L. Chiang, E-mail: vincent_chiang{at}ncsu.edu


  Abstract

We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the most amenable explants to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by GUS staining. Regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as short as five months after co-cultivation. This system may help facilitate studies of gene functions in tree growth and development at a genome level.

Keywords: Agrobacterium tumefaciens; Black cottonwood; Functional genomics; Genetic transformation; Populus trichocarpa; Nisqually-1.
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