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Plant and Cell Physiology Advance Access published online on September 20, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl012
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular Paper

Identification of active transposon dTok, a member of hAT family, in rice

Sunok Moon 1, Ki-Hong Jung 1, Do-eun Lee 1, Wen-Zhu Jiang 2, Hee Jong Koh 2, Mun-Hue Heu 2, Dong Sun Lee 3, Hak Soo Suh 4, and Gynheung An 5 *

1 National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea
2 Laboratory of Rice Genomics and Breeding, College of Agriculture and Life Science, Seoul National University, Seoul 151-921, Republic of Korea
3 Institute of Biotechnology, Yeungnam University, Kyongsan 712-749, Republic of Korea
4 School of Biological Resources, College of Natural Resources, Yeungnam University, Kyongsan 712-749, Republic of Korea
5 National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea; Functional Genomics Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea

* To whom correspondence should be addressed.
Gynheung An, E-mail: genean{at}postech.ac.kr


  Abstract

Recent completion of sequencing for the rice genome has revealed that it contains over 40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, homologue to CLAVATA1. Insertion of the element into FON1 generated an 8-bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out from the FON1 gene, leaving 5- to 8-bp footprints. And reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar 'Nipponbare' contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase.

Keywords: hAT superfamily; floral organ number 1/multiple pistil 2; Oryza; rice; transposon.
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