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Plant and Cell Physiology Advance Access published online on September 16, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl010
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Short Communication

Repression of Nitrate Uptake by Replacement of Asp-105 by Asn in AtNRT3.1 in Arabidopsis thaliana L

Tahei Kawachi 1 *, Yoshihito Sunaga 1, Munehiro Ebato 1, Tetsuya Hatanaka 1, and Hisatomi Harada 2

1 National Institute of Livestock and Grassland Science, 768, Senbonmatsu, Nasushiobara, Tochigi, 329-2793 Japan
2 National Institute of Livestock and Grassland Science, 768, Senbonmatsu, Nasushiobara, Tochigi, 329-2793 Japan; Present address Ogata farm, Agricultural Experiment Station, Akita Prefectural Agriculture, Forestry and Fisheries Research Center, Higashi 1-1, Ogata, Minami-Akita, Akita, 010-0442 Japan

* To whom correspondence should be addressed.
Tahei Kawachi, E-mail: tahei{at}affrc.go.jp


  Abstract

An Arabidopsis mutant (rnc1) with a mutation at the 313th nucleotide from the translational start site of AtNRT3.1 was isolated. The mutation resulted in the replacement of Asp by Asn at the 105th amino acid in a region conserved among higher plants. In the rnc1 mutant, both the nitrate concentrations in plants and the nitrate uptake from the medium were less than 13% compared to those of the wild type, while AtNRT3.1 mRNA was accumulated similarly and both AtNRT1.1 and AtNRT2.1 mRNA were decreased. These results suggest that the replacement of Asp-105 in AtNRT3.1 markedly reduces nitrate uptake and accumulation.

Keywords: Arabidopsis thaliana; a single nucleotide mutation; AtNRT3.1; nitrate uptake.
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