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Plant and Cell Physiology Advance Access published online on September 16, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl009
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular Paper

Gene trapping in Arabidopsis reveals genes involved in vascular development

Shingo Nagawa 1 *, Shinichirou Sawa 1, Shusei Sato 2, Tomohiko Kato 3, Satoshi Tabata 2, and Hiroo Fukuda 1 *

1 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
2 Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari Kisarazu, Chiba 292-0818, Japan
3 Forestry Research Institute, Oji Paper Company, 24-9 Nobono-cho Kameyama, Mie, 519-0212 Japan

* To whom correspondence should be addressed.
Shingo Nagawa, E-mail: nagawa{at}biol.s.u-tokyo.ac.jp
Hiroo Fukuda, E-mail: fukuda{at}biol.s.u-tokyo.ac.jp


  Abstract

The procambium is made up of stem cells that give rise to various vascular cells in plants. To understand the molecular nature of procambium cells, we tried to identify genes that characterize procambium cells using Arabidopsis gene trap lines. Among 26,000 gene trap lines, we found 67 lines in which {beta}-glucuronidase (GUS) staining occurred along vascular tissues in cotyledons and/or adult leaves. Although 4 gene trap lines showed procambium-preferential GUS expression, their expression patterns differed from each other during procambium development in root tips and young rosette leaves. Genomic regions flanking the gene trap insertion points in 25 of the 67 lines were determined, including three lines showing preferential-GUS staining of the procambium. The three procambium-related genes encoded PINHEAD, katanin, and an unknown DUF740 domain-containing protein. We discuss procambium development based on the functions and the differential GUS staining patterns of the procambium-related genes.

Keywords: Arabidopsis thaliana; beta-glucuronidase; gene expression pattern; gene trap lines; procambium; vascular development.
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