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Plant and Cell Physiology Advance Access published online on August 30, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl003
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular Paper

Differential Expression of Two Flavonoid 3'-hydroxylase cDNAs Involved in Biosynthesis of Anthocyanin Pigments and 3-Deoxyanthocyanidin Phytoalexins in Sorghum

Chun-Hat Shih 1, Ivan K Chu 2, Wing Kin Yip 3, and Clive Lo 3 *

1 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China;Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China
2 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China
3 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China

* To whom correspondence should be addressed.
Clive Lo, E-mail: clivelo{at}hkucc.hku.hk


  Abstract

Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2, and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed more than 60% identity to the Arabidopsis TT7 (F3'H) protein. Over-expression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylaed flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represents a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.

Keywords: Flavonoid 3'-hydroxylases; 3-Deoxyanthocyanidins; Anthocyanins; Sorghum.
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