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Plant and Cell Physiology Advance Access published online on August 23, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl002
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Regular Article

Sequence Upstream of the Wheat (Triticum aestivum L.) ALMT1 Gene and Its Relationship to Aluminum Resistance

Takayuki Sasaki 1, Peter R. Ryan 2, Emmanuel Delhaize 2, Diane M. Hebb 2, Yasunari Ogihara 3, Kanako Kawaura 3, Kazuhiro Noda 1, Toshio Kojima 4, Atsushi Toyoda 4, Hideaki Matsumoto 1, and Yoko Yamamoto 1 *

1 Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, Okayama 710-0046, Japan
2 CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia
3 Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, Yokohama, Kanagawa 244-0813, JAPAN
4 RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, JAPAN

* To whom correspondence should be addressed.
Yoko Yamamoto, E-mail: yoko{at}rib.okayama-u.ac.jp


  Abstract

Aluminum (Al) resistance in wheat relies on the Al-activated malate efflux from root apices, which appears to be controlled by an Al-activated anion transporter encoded by the ALMT1 gene on chromosome 4DL. Genomic regions upstream and downstream of ALMT1 in 69 wheat lines were characterized to identify patterns that might influence ALMT1 expression. The first 1,000-bp downstream of ALMT1 was reasonably well conserved among the lines examined apart from the presence of a transposon-like sequence which was did not associated correlate with Al resistance. By contrast, the first 1,000-bp upstream of the ALMT1 coding region was more variable and six different patterns could be discerned (Types I to VI). Type I had the simplest structure while the others had blocks of sequence that were duplicated or triplicated in different arrangements. A pattern emerged among the lines of non-Japanese origin such that the number of repeats in this upstream region was positively correlated with the levels of ALMT1 expression and Al resistance. By contrast many of the Japanese lines exhibited a large variation in ALMT1 expression and Al resistance despite possessing the same Type of upstream region. Although ALMT1 expression was also poorly correlated with Al-activated malate efflux in the Japanese lines, a strong correlation between malate efflux and Al resistance suggested that malate efflux was still the primary mechanism for Al resistance, and that additional genes are involved in the post-transcriptional regulation of ALMT1 function.

Keywords: ALMT1 gene; aluminium resistance; genomic upstream sequence; malate transporter; Triticum aestivum L.

DDBJ accession numbers of ALMT1 upstream sequences:AB243162 for Type I, AB243163 for Type II, AB243164 for Type III from a TAC clone sequence of Chinese Spring, AB243165 for Type IV, AB243166 for Type V, AB243167 for Type VI, AB243168 for Type I with several SNPs (Type I’) of wheat cultivar Clark's cream, AB243169 for Type I with several SNPs (Type I’) of wheat cultivar Cranbrook, AB243170 for Type I with several SNPs (Type I’) of Aegilops tauschii


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