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Plant and Cell Physiology Advance Access published online on August 22, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcl001
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Short Communication

Arabidopsis Tubulin Folding Cofactor B interacts with {alpha} tubulin in vivo

Pankaj Dhonukshe 1 *, Bastiaan O. R. Bargmann 2, and Theodorus W. J. Gadella Jr 3 *

1 Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands; Current Address: Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 72076, Tübingen, Germany
2 Section of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands; Current Address: New York University, Dept. of Biology, 100 Washington Square East 1009, Silver Building New York, NY 10003, USA
3 Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands

* To whom correspondence should be addressed.
Pankaj Dhonukshe, E-mail: pankaj.dhonukshe{at}zmbp.uni-tuebingen.de
Theodorus W. J. Gadella Jr, E-mail: gadella{at}science.uva.nl


  Abstract

Microtubule biogenesis requires {alpha}{beta}-tubulin dimers that are generated from {alpha} and {beta} tubulin following posttranslational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The sub-cellular localization of AtTFCB is mainly cytosolic. AtTFCB overexpressing cells have less microtubules as compared to the controls. Multi-mode Fluorescence Resonance Energy Transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with {alpha} tubulin in live plant cells. We conclude that AtTFCB interacts with {alpha} tubulin in vivo and its overexpression reduces the number of microtubules.

Keywords: cofactor; GFP; FRET; microtubule; tubulin folding.
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