Skip Navigation



Plant and Cell Physiology Advance Access published online on August 3, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj095
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/9/1251    most recent
pcj095v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ishizuka, T.
Right arrow Articles by Ikeuchi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishizuka, T.
Right arrow Articles by Ikeuchi, M.
Agricola
Right arrow Articles by Ishizuka, T.
Right arrow Articles by Ikeuchi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received May 6, 2006
Accepted July 25, 2006

Regular Paper

Characterization of cyanobacteriochrome TePixJ from a thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1

Takami Ishizuka 1, Takashi Shimada 2, Koji Okajima 1, Shizue Yoshihara 3, Yuriko Ochiai 1, Mitsunori Katayama 1, and Masahiko Ikeuchi 1 *

1 Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan
2 Tsukuba Proteomics Research Center, SHIMADZU BIOTECH, 3-17-1, Azuma, Tsukuba 305-0031, Japan
3 Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan

* To whom correspondence should be addressed.
Masahiko Ikeuchi, E-mail: mikeuchi{at}bio.c.u-tokyo.ac.jp


  Abstract

A putative photoreceptor gene, TepixJ, of a thermophilic cyanobacterium is homologous to SypixJ1 that mediates positive phototaxis in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. The putative chromophore-binding GAF domain of TePixJ protein was overexpressed as a fusion with a poly-histidine tag (His-TePixJ_GAF) in Synechocystis cells and isolated into homogeneity. The photoreversible conversion of the His-TePixJ_GAF showed peaks at 531 nm, 341 nm and 266 nm for the green light-absorbing form (Pg form) and peaks at 433 nm and 287 nm for the blue light-absorbing form (Pb form). At 77K, the Pg form fluoresced at 580 nm, while the Pb form did not emit any fluorescence. Mass spectrometry of the tryptic chromopeptide demonstrated that a phycocyanobilin isomer binds to the conserved cysteine at the ring A via a thioether bond. It is established that TePixJ and SyPixJ1 are novel photoreceptors in cyanobacteria ("cyanobacteriochrome") that are similar but distinct from the phytochrome and bacteriophytochromes.

Keywords: cyanobacteriochrome; GAF domain; photoreceptor; phototaxis; phycocyanobilin; phytochrome.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. T. Ulijasz, G. Cornilescu, D. von Stetten, C. Cornilescu, F. Velazquez Escobar, J. Zhang, R. J. Stankey, M. Rivera, P. Hildebrandt, and R. D. Vierstra
Cyanochromes Are Blue/Green Light Photoreversible Photoreceptors Defined by a Stable Double Cysteine Linkage to a Phycoviolobilin-type Chromophore
J. Biol. Chem., October 23, 2009; 284(43): 29757 - 29772.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Hirose, T. Shimada, R. Narikawa, M. Katayama, and M. Ikeuchi
Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein
PNAS, July 15, 2008; 105(28): 9528 - 9533.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Ishizuka, R. Narikawa, T. Kohchi, M. Katayama, and M. Ikeuchi
Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus Harbors Phycoviolobilin as a Chromophore
Plant Cell Physiol., September 1, 2007; 48(9): 1385 - 1390.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.