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Plant and Cell Physiology Advance Access published online on August 3, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj093
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© The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received June 17, 2006
Accepted July 21, 2006

Regular Paper

The Poplar Glycosyltransferase GT47C is Functionally Conserved with Arabidopsis Fragile Fiber8

Gong-Ke Zhou 1, Ruiqin Zhong 1, Elizabeth A. Richardson 1, W. Herbert Morrison III 2, C. Joseph Nairn 3, Alicia Wood-Jones 3, and Zheng-Hua Ye 1 *

1 Department of Plant Biology, University of Georgia, Athens, GA 30602 U.S.A
2 Richard B. Russell Agriculture Research Center, U.S. Department of Agriculture, Agriculture Research Service, Athens, GA 30604, U.S.A
3 Danial B. Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, U.S.A

* To whom correspondence should be addressed.
Zheng-Hua Ye, E-mail: zhye{at}plantbio.uga.edu


  Abstract

Xylan is the major hemicellulose in the dicot wood. Unraveling genes involved in the biosynthesis of xylan will be of importance in understanding the process of wood formation. In this report, we investigated the possible role of poplar GT47C, a glycosyltransferase belonging to family GT47, in the biosynthesis of xylan. PoGT47C from the hybrid poplar Populus alba x tremula exhibits 84% sequence similarity to Fragile fiber8 (FRA8), which is involved in the biosynthesis of glucuronoxylan in Arabidopsis. Phylogenetic analysis of glycosyltransferase family GT47 in the Populus trichocarpa genome revealed that GT47C is the only closest homolog of FRA8. In situ hybridization showed that the PoGT47C gene was expressed in developing primary xylem, secondary xylem, and phloem fibers of stems, and in developing secondary xylem of roots. Sequence analysis suggested that PoGT47C is a type II membrane protein and subcellular localization study demonstrated that fluorescent protein-tagged PoGT47C was located in Golgi. Immunolocalization with a xylan monoclonal antibody LM10 revealed a nearly complete loss of xylan signals in the secondary walls of fibers and vessels in the Arabidopsis fra8 mutant. Expression of PoGT47C in the fra8 mutant restored the secondary wall thickness and xylan content to the wild-type level. Together, these results suggest that PoGT47C is functionally conserved with FRA8 and it is likely involved in xylan synthesis during wood formation.

Keywords: Arabidopsis thaliana; Glycosyltransferase; Poplar; Secondary wall synthesis; Xylan.
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