Skip Navigation



Plant and Cell Physiology Advance Access published online on July 18, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj089
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/8/1146    most recent
pcj089v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ivanov, A. G.
Right arrow Articles by Huner, N. P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ivanov, A. G.
Right arrow Articles by Huner, N. P. A.
Agricola
Right arrow Articles by Ivanov, A. G.
Right arrow Articles by Huner, N. P. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received March 22, 2006
Accepted July 3, 2006

Regular Paper

Digalactosyl-diacylglycerol deficiency impairs the capacity for photosynthetic intersystem electron transport and state transitions in Arabidopsis thaliana due to photosystem I acceptor-side limitations

Alexander G. Ivanov 1, Luke Hendrickson 2, Marianna Krol 3, Eva Selstam 2, Gunnar Öquist 2, Vaughan Hurry 2, and Norman P. A. Huner 3 *

1 Department of Biology and The Biotron, University of Western Ontario, London, Ontario, Canada N6A 5B7; Umea Plant Science Centre, Department of Plant Physiology, University of Umeå, Umeå S-901 87, Sweden
2 Umea Plant Science Centre, Department of Plant Physiology, University of Umeå, Umeå S-901 87, Sweden
3 Department of Biology and The Biotron, University of Western Ontario, London, Ontario, Canada N6A 5B7

* To whom correspondence should be addressed.
Norman P. A. Huner, E-mail: nhuner{at}uwo.ca


  Abstract

Compared to wild type, the dgd1 mutant of Arabidopsis thaliana exhibited a lower amount of PSI-related chlorophyll-protein complexes and lower abundance of the PSI-associated polypeptides, PsaA, PsaB, PsaC, PsaL and PsaH with no changes in the levels of Lhca1-4. Functionally, the dgd1 mutant exhibited a significantly lower light-dependent, steady state oxidation level of P700 (P700+) in vivo a higher intersystem electron pool size, restricted linear electron transport and a higher rate of reduction of P700+ in the dark indicating an increased capacity for PSI cyclic electron transfer compared to wild type. Concomitantly, the dgd1 mutant exhibited a higher sensitivity to and incomplete recovery of photoinhibition of PSI. Furthermore, dgd1 exhibited a lower capacity to undergo state transitions compared to wild type which was associated with a higher reduction state of the plastoquinone (PQ) pool. We conclude that DGDG deficiency results in PSI acceptor-side limitations that alter the flux of electrons through the photosynthetic electron chain and impair the regulation of distribution of excitation energy between the photosystems. These results are discussed in terms of thylakoid membrane domain reorganization in response to DGDG deficiency in Arabidopsis thaliana.

Keywords: Arabidopsis dgd1 mutant; Chlorophyll fluorescence; Digalactosyl-diacylglycerol; Photoinhibition; P700; State transitions.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Kobayashi, M. Kondo, H. Fukuda, M. Nishimura, and H. Ohta
Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis
PNAS, October 23, 2007; 104(43): 17216 - 17221.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.