Plant and Cell Physiology Advance Access published online on July 22, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj087
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1 Institut für Biologie II/Botanik, Albert Ludwigs Universität, Schänzlestrasse 1, D-79104 Freiburg, Germany
* To whom correspondence should be addressed. The phytochrome family of red/far-red (R/FR) photoreceptors is involved in the regulation of a wide range of developmental responses in plants. The Arabidopsis genome contains five phytochromes (phyA-E) among which phyA and phyB play the most important roles. Phytochromes localise to the cytosol in the dark and accumulate in the nucleus under light conditions inducing specific phytochrome mediated responses. Light-regulated nuclear accumulation of the phytochrome photoreceptors is therefore considered a key regulatory step of these pathways. In fact, one of the most severe phyA signalling mutants, fhy1 (far red elongated hypocotyl 1) is strongly affected in nuclear accumulation of phyA. The fhy1 fhl (fhy1 like) double mutant, lacking both FHY1 and its only close homolog FHL, is virtually blind to far-red light like phyA null seedlings. Here we show that FHL accounts for residual amounts of phyA in the nucleus in fhy1 background and that nuclear accumulation of phyA is completely inhibited in an fhy1 FHL RNAi knock down line. Moreover, we demonstrate that FHL and phyA interact with each other in a light dependent manner and that they colocalise in light induced nuclear speckles. We also identify a phyA binding site at the C-terminus of FHY1 and FHL and show that the N-terminal 406 amino acids of phyA are sufficient for the interaction with FHY1/FHL.
Received May 25, 2006
Accepted July 1, 2006
Rapid Paper
FHY1 and FHL act together to mediate nuclear accumulation of the phytochrome A photoreceptor
Andreas Hiltbrunner 1, Anke Tscheuschler 1, András Viczián 1, Tim Kunkel 1, Stefan Kircher 1, and Eberhard Schäfer 1 *
Eberhard Schäfer, E-mail: eberhard.schaefer{at}biologie.uni-freiburg.de
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