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Plant and Cell Physiology Advance Access published online on July 18, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj086
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© The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received February 7, 2006
Accepted July 1, 2006

Short Communication

Purification and Characterization of Plant Dynamin from tobacco BY-2 cells

Takahiro Hamada 1, Hisako Igarashi 2, Maki Yao 3, Takashi Hashimoto 3, Teruo Shimmen 1, and Seiji Sonobe 1 *

1 Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297, Japan
2 Plant Science Center, RIKEN, 1-7-22 Suehiro, Turumi-ku, Yokohama-shi, Kanagawa, 230-0043, Japan
3 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan

* To whom correspondence should be addressed.
Seiji Sonobe, E-mail: sonobe{at}sci.u-hyogo.ac.jp


  Abstract

We purified an 84 kDa polypeptide from MAPs (microtubule-associated proteins) fraction of tobacco BY-2 cultured cells. LC/MS/MS (liquid chromatography-tandem mass spectrometry) analysis revealed that this polypeptide is a tobacco homologue of AtDRP3 (Arabidopsis thaliana dynamin-related protein 3). Electron microscopy revealed that NtDRP3 (Nicotiana tabacum dynamin-related protein 3) assembles to form filamentous structure. When GDP was added to NtDRP3 fraction, the filaments disappeared and many particles appeared. Biochemical analysis revealed that NtDRP3 could bind and bundle both microtubules and actin filaments in vitro.

Keywords: Actin filament; Dynamin; Microtubule; Microtubule-associated protein (MAP); NtDRP3; Tobacco BY-2 cell.
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