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Plant and Cell Physiology Advance Access published online on July 18, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj083
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© The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 27, 2006
Accepted June 26, 2006

Regular Paper

FtsZ characterization and immunolocalization in the two phases of plastid reorganization in arbuscular mycorrhizal roots of Medicago truncatula

Swanhild Lohse 1, Bettina Hause 1, Gerd Hause 1, and Thomas Fester 1 *

1 Leibniz Institute of Plant Biochemistry, Department of Secondary Metabolism, Weinberg 3, D-06120 Halle (Saale), Germany; Biocenter, University of Halle, Weinbergweg 22, D-06120 Halle (Saale), Germany

* To whom correspondence should be addressed.
Thomas Fester, E-mail: tfester{at}ipb-halle.de


  Abstract

We have analyzed plastid proliferation in root cortical cells of Medicago truncatula colonized by arbuscular mycorrhizal (AM) fungi by concomitantly labelling fungal structures, root plastids, a protein involved in plastid division (FtsZ1) and a protein involved in the biosynthesis of AM-specific apocarotenoids. Antibodies directed against FtsZ1 have been generated after heterologous expression of the respective gene from M. truncatula and characterization of the gene product. Analysis of enzymatic activity and assembly experiments showed similar properties of this protein when compared to the bacterial proteins. Immunocytological experiments allowed to clearly differentiate two phases of fungal and plastid development and to monitor plastid division during these phases. In the early phase of arbuscule development, lens-shaped plastids, intermingled between the arbuscular branches, are frequently dividing. Arbuscule degradation, in contrast, is characterized by large, tubular plastids, decorated by a considerable number of FtsZ division rings.

Keywords: Plastid division; FtsZ; arbuscular mycorrhiza; Medicago truncatula; arbuscule senescence.
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