Plant and Cell Physiology Advance Access published online on July 2, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj078
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Laboratory of Plant Molecular Physiology, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan
* To whom correspondence should be addressed. Temperature is a primary environmental cue for seed germination of many weeds and vegetables. To investigate the mechanism of germination regulation by temperature, we selected five high temperature (thermoinhibition) resistant germination mutants (TRW lines) from 20,000 T-DNA insertion lines of Arabidopsis. Segregation analyses indicated that each of the five lines had single locus recessive mutations. The seeds of TRW134-15 and TRW187 showed reduced sensitivity to ABA and also GA biosynthesis inhibitor, paclobutrazol. Genetic and nucleotide sequencing analyses indicated that TRW187 is a new allele of abi3 (abi3-14). TRW71-1 exhibited a maternal effect for both thermoinhibition resistant and transparent testa phenotypes, and genetic analysis revealed that the mutation was allelic to tt7 (tt7-4 sib). Interestingly, the seeds of reduced dormancy mutants rdo1, rdo2, rdo3 and rdo4 were also thermoinhibition tolerant, and all the TRW seeds showed reduced dormancy. As rdo3, TRW13-1 had shorter siliques and slightly shorter stems than the wild type. The mutation of TRW13-1 was mapped on the bottom arm of chromosome 1 where rdo3 has also been mapped, but the two mutants are not allelic. We designated TRW13-1 as thermoinhibition resistant germination 1 (trg1). We also mapped the ABA insensitive mutation of TRW134-15 to the bottom arm of chromosome 5 and named as trg2. These results show that both embryo/endosperm and maternal factors contribute to germination inhibition at supraoptimal temperature in Arabidopsis. In addition, we confirm the role of ABA in thermoinhibition of seed germination and a link between seed physiological dormancy and response to high temperature.
Received January 17, 2006
Accepted June 13, 2006
Regular Paper
Isolation and characterization of high temperature resistant germination mutants of Arabidopsis thaliana
Noriko Tamura 1,
Takahiro Yoshida 2,
Arata Tanaka 1,
Ryuta Sasaki 3,
Asuka Bando 4,
Shigeo Toh 1,
Loïc Lepiniec 5,
and
Naoto Kawakami 1 *
2 Laboratory of Plant Molecular Physiology, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan; Miyoshi Breeding Station, Musashino Seed Co., Ltd., Iruma-gun 354-0045, Japan
3 Laboratory of Plant Molecular Physiology, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8577, Japan
4 Laboratory of Plant Molecular Physiology, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan; Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan
5 Laboratoire de Biologie des Semences, Unite Mixte de Recherche 204, Institut National de la Recherche Agronomique/Institut National Agronomique Paris-Grignon, Institut Jean-Pierre Bourgin, 78026 Versailles, France
Naoto Kawakami, E-mail: kawakami{at}isc.meiji.ac.jp
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Q. Bu, H. Li, Q. Zhao, H. Jiang, Q. Zhai, J. Zhang, X. Wu, J. Sun, Q. Xie, D. Wang, et al. The Arabidopsis RING Finger E3 Ligase RHA2a Is a Novel Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development Plant Physiology, May 1, 2009; 150(1): 463 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Argyris, P. Dahal, E. Hayashi, D. W. Still, and K. J. Bradford Genetic Variation for Lettuce Seed Thermoinhibition Is Associated with Temperature-Sensitive Expression of Abscisic Acid, Gibberellin, and Ethylene Biosynthesis, Metabolism, and Response Genes Plant Physiology, October 1, 2008; 148(2): 926 - 947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Toh, A. Imamura, A. Watanabe, K. Nakabayashi, M. Okamoto, Y. Jikumaru, A. Hanada, Y. Aso, K. Ishiyama, N. Tamura, et al. High Temperature-Induced Abscisic Acid Biosynthesis and Its Role in the Inhibition of Gibberellin Action in Arabidopsis Seeds Plant Physiology, March 1, 2008; 146(3): 1368 - 1385. [Abstract] [Full Text] [PDF]
|
|