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Plant and Cell Physiology Advance Access published online on June 9, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj071
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received February 19, 2006
Accepted May 26, 2006

Regular Paper

The Expression of Different Superoxide Dismutase Forms Is Cell-Type Dependent in Olive (Olea europaea L.) Leaves

Francisco J. Corpas 1 *, Ana Fernández-Ocaña 2, Alfonso Carreras 2, Raquel Valderrama 2, Francisco Luque 2, Francisco J. Esteban 2, María Rodríguez-Serrano 1, Mounira Chaki 2, Jóse R. Pedrajas 2, Luisa M. Sandalio 1, Luis A. del Río 1, and Juan B. Barroso 2

1 Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (EEZ), CSIC, Granada, Spain
2 Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (EEZ), Área de Bioquímica y Biología Molecular, Universidad de Jaén, Spain

* To whom correspondence should be addressed.
Francisco J. Corpas, E-mail: javier.corpas{at}eez.csic.es


  Abstract

Superoxide dismutase is a key antioxidant enzyme present in prokaryotic and eukaryotic cells as a first line of defence against the accumulation of superoxide radicals. In olive leaves, the enzymatic system superoxide dismutase (SOD) was characterized and was composed by three isozymes, a Mn-SOD, an Fe-SOD and a CuZn-SOD. Transcript expression analysis of whole leaves showed that the three isozymes represented 82 %, 17 % and 0.8 % of the total SOD expressed, respectively. Using the combination of Laser Capture Microdissection (LCM) and real time quantitative RT-PCR, the expression of these SOD isozymes was studied in different cell types of olive leaves, including spongy mesophyll, palisade mesophyll, xylem, and phloem. In spongy mesophyll cells, the isozyme proportion was similar to that in whole leaves, but in the other cells the proportion of expressed SOD isozymes was different. In palisade mesophyll cells, Fe-SOD was the most abundant, followed by Mn-SOD and CuZn-SOD, but in phloem cells Mn-SOD was the most prominent isozyme, and Fe-SOD was present in trace amounts. In xylem cells, only the Mn-SOD was detected. On the other hand, the highest accumulation of superoxide radicals was localized in vascular tissue which was the tissue with the lowest level of SOD transcripts. These data show that in olive leaves each SOD isozyme has a different gene expression depending on the cell type of the leaf.

Keywords: confocal laser scanning microscopy (CLSM); quantitative PCR; laser capture microdissection (LCM); olive; Olea europaea L.; palisade mesophyll; phloem; SOD expression; spongy mesophyll; superoxide radicals; SOD; superoxide dismutase; xylem.
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