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Plant and Cell Physiology Advance Access published online on June 7, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj066
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received March 24, 2006
Accepted May 21, 2006

Regular Paper

Hormone Interactions and Regulation of Unifoliata, PsPK2, PsPIN1 and LE Gene Expression in Pea (Pisum sativum) Shoot Tips

Fang Bai 1 and Darleen A. DeMason 1 *

1 Botany and Plant Sciences, University of California, Riverside, CA 92521

* To whom correspondence should be addressed.
Darleen A. DeMason, E-mail: demason{at}ucrac1.ucr.edu


  Abstract

The Unifoliata (Uni) gene plays a major role in development of the compound leaf in pea, but its regulation is unknown. In this study, we examined effects of plant hormones on the expression of Uni, PsPK2 (the gene for a pea homologue of Arabidopsis PID, a regulator of PIN1 targeting), PsPIN1 (the major gene for a putative auxin efflux carrier), and LE (a gibberellin biosynthesis gene, GA3ox), and also examined mutual hormonal regulation of these genes, in pea shoot tips, including a number of mutants. The Uni promoter possessed putative auxin- and gibberellin-response elements. mRNA levels of PsPIN1 were increased in afila, which replaces leaflets with branched tendrils; and reduced in tendrilless, which replaces tendrils with leaflets compared to WT. By contrast, mRNA levels of LE were increased in uni and tendrilless and decreased in afila compared to WT. Uni, PsPK2 and PsPIN1 are positively regulated by gibberellin and auxin, and were induced to higher levels by simultaneous application of auxin and gibberellin. Auxin-induction of Uni, PsPK2, and PsPIN1 did not require de novo protein synthesis. LE was positively regulated by auxin and cytokinin. In conclusion, these results support the hypothesis that auxin and GA positively regulate Uni, which controls pea compound leaf development. Also, Uni, PsPIN1, PsPK2 and LE are differentially expressed in the leaf mutants, suggesting that mutual regulation by auxin and GA promote compound leaf development.

Keywords: Auxin; Gibberellin; Leaf development; Pea; Pisum; Unifoliata.
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