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Plant and Cell Physiology Advance Access published online on May 26, 2006

Plant and Cell Physiology, doi:10.1093/pcp/pcj061
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 17, 2006
Accepted May 10, 2006

Short Communication

Constitutive Accumulation of cis-Piceid in Transgenic Arabidopsis Over-Expressing a Sorghum Stilbene Synthase Gene

Christine K. Y. Yu 1, Corey N. W. Lam 2, Karin Springob 3, Jüergen Schmidt 3, Ivan K. Chu 4, and Clive Lo 5 *

1 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China
2 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China
3 Leibniz-Institut für Pflanzenbiochemie, 06120 Halle/Saale, Germany
4 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Genome Research Centre, The University of Hong Kong, Pokfulam Road, Hong Kong, China
5 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China

* To whom correspondence should be addressed.
Clive Lo, E-mail: clivelo{at}hkucc.hku.hk


   Abstract

Sorghum SbSTS1 was the first example of a stilbene synthase gene in monocots. Previously, we demonstrated that the gene was involved in defense responses. To examine its biochemical function in planta, SbSTS1 was over-expressed in transgenic Arabidopsis. Metabolite analysis revealed that cis-resveratrol glucoside (piceid) accumulated as the major stilbene in the transgenic lines. Using LC-MS/MS in selected reaction monitoring mode, up to 580 µg/g fresh weight of cis-piceid were detected in 2-week-old plants, which represent a convenient source of the cis-isomers for pharmacological investigations. Our results also suggested the presence of unknown stilbene isomerase activities in Arabidopsis.

Keywords: cis-resveratrol; cis-piceid; stilbene synthase; sorghum; Arabidopsis.
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