Plant and Cell Physiology Advance Access published online on May 26, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj061
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1 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China
* To whom correspondence should be addressed. Sorghum SbSTS1 was the first example of a stilbene synthase gene in monocots. Previously, we demonstrated that the gene was involved in defense responses. To examine its biochemical function in planta, SbSTS1 was over-expressed in transgenic Arabidopsis. Metabolite analysis revealed that cis-resveratrol glucoside (piceid) accumulated as the major stilbene in the transgenic lines. Using LC-MS/MS in selected reaction monitoring mode, up to 580 µg/g fresh weight of cis-piceid were detected in 2-week-old plants, which represent a convenient source of the cis-isomers for pharmacological investigations. Our results also suggested the presence of unknown stilbene isomerase activities in Arabidopsis.
Received April 17, 2006
Accepted May 10, 2006
Short Communication
Constitutive Accumulation of cis-Piceid in Transgenic Arabidopsis Over-Expressing a Sorghum Stilbene Synthase Gene
Christine K. Y. Yu 1,
Corey N. W. Lam 2,
Karin Springob 3,
Jüergen Schmidt 3,
Ivan K. Chu 4,
and
Clive Lo 5 *
2 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China
3 Leibniz-Institut für Pflanzenbiochemie, 06120 Halle/Saale, Germany
4 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China; Genome Research Centre, The University of Hong Kong, Pokfulam Road, Hong Kong, China
5 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, China
Clive Lo, E-mail: clivelo{at}hkucc.hku.hk
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