Skip Navigation



Plant and Cell Physiology Advance Access published online on May 16, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj053
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/7/818    most recent
pcj053v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lehner, A.
Right arrow Articles by Bailly, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lehner, A.
Right arrow Articles by Bailly, C.
Agricola
Right arrow Articles by Lehner, A.
Right arrow Articles by Bailly, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received January 26, 2006
Accepted April 24, 2006

Regular Paper

Changes in Lipid Status and Glass Properties in Cotyledons of Developing Sunflower Seeds

Arnaud Lehner 1, Françoise Corbineau 1, and Christophe Bailly 1 *

1 Université Pierre et Marie Curie-Paris 6, Physiologie Végétale Appliquée, Le Raphaël, site d'Ivry, boîte 152, 4 place Jussieu, Paris, F-75005 France

* To whom correspondence should be addressed.
Christophe Bailly, E-mail: bailly{at}ccr.jussieu.fr


  Abstract

Biochemical events involved in the acquisition of germinability and storability during orthodox seed development are well documented, however the roles played by the physical organization of lipids and water are poorly characterized. The aim of this work was to determine, using a thermodynamic approach, whether changes in thermal properties of lipid reserves, water and intracellular glasses might play a role in sunflower (Helianthus annuus L.) seed development. Triacyglycerols (TAG) accumulated in cotyledons until the end of seed filling which occurred 42 days after anthesis (DAA). Further seed development, leading to mature seed at 58 DAA, was mainly associated with an enlargement of lipid bodies without significant changes either in the lipid content nor in their composition. When cooled to -100°C, lipid reserves from cotyledons of mature seeds displayed {alpha} and {beta}' polymorphic crystalline structures, however the ability to form {alpha} crystals, which was an indicator of lipid purity, progressively appeared during seed development. Characteristics of lipid melting confirmed that seed maturation drying was associated with changes in TAG physical organization. Cotyledon development was associated with an increase in the temperature of glass to rubber transition (Tg), thus suggesting a decrease in molecular mobility during maturation drying. This phenomenon was concomitant with an increase in raffinose content. Our results demonstrate that physical characteristics of lipid reserves and glasses of sunflower cotyledons are developmentally regulated and might play a role in acquisition of seed germinability and storability.

Keywords: Differential Scanning Calorimetry; Glass; Seed development; Seed vigour; Sunflower; Triacylglycerol.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.