Plant and Cell Physiology Advance Access first published online on April 17, 2006
This version published online on April 21, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj051
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1 Faculty of Integrated Arts & Sciences, Hiroshima University, 1-7-1 Kagamiyama, Higashi-hiroshima, 739-8521 Japan
* To whom correspondence should be addressed. To elucidate the cellulose synthesis mechanism, we isolated a mutant of Arabidopsis (css1) that showed changed sensitivity to cellulose biosynthesis inhibitor. The analysis of phenotypes indicated that the css1 mutation influenced various fundamental metabolisms including amino acid metabolism, triacylglycerol degradation and polysaccharide synthesis (cellulose and starch) during early stage of plant growth. Unexpectedly the map-based cloning of the responsible gene for the css1 mutation identified a protein (At-nMat1a) that was assumed a splicing factor of mitochondrial group II intron. In accordance with this result, this mutant exhibited improper splicing of mitochondrial NAD4 transcript. We noticed that the phenotypes of css1 mutant are similar to the responses to anoxia that hinders mitochondrial aerobic respiration. We seem that the defect in the function of mitochondria influences various aspects of cellular fundamental metabolism including cellulose synthesis. Our results suggested that sucrose synthase (SuSy), an enzyme involved in the biosynthesis of cellulose, plays key roles in the connection between mitochondria and cellulose synthesis. The isolation of css1 mutant also provides a useful resource in the study of post-transcriptional gene regulation in mitochondria.
Received June 24, 2005
Accepted April 8, 2006
Regular Paper
A Mutation in At-nMat1a, Which Encodes a Nuclear Gene Having High Similarity to Group II Intron Maturase, Causes Impaired Splicing of Mitochondrial NAD4 Transcript and Altered Carbon Metabolism in Arabidopsis thaliana
Naoki Nakagawa 1 *
and
Naoki Sakurai 1
Naoki Nakagawa, E-mail: naka{at}hiroshima-u.ac.jp
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