Skip Navigation



Plant and Cell Physiology Advance Access published online on April 11, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj045
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/6/736    most recent
pcj045v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Boyko, A.
Right arrow Articles by Kovalchuk, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boyko, A.
Right arrow Articles by Kovalchuk, I.
Agricola
Right arrow Articles by Boyko, A.
Right arrow Articles by Kovalchuk, I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received January 20, 2006
Accepted March 31, 2006

Regular Paper

Increase of Homologous Recombination Frequency in Vascular Tissue of Arabidopsis Plants Exposed to Salt Stress

Alex Boyko 1, Darryl Hudson 2, Prasanna Bhomkar 1, Palak Kathiria 1, and Igor Kovalchuk 1 *

1 Department of Biological Sciences, University of Lethbridge, Lethbridge, AB. T1K 3M4, Canada
2 Department of Biological Sciences, University of Lethbridge, Lethbridge, AB. T1K 3M4, Canada; Current address: Dept. of Molecular and Cellular Biology (MCB), University of Guelph, Axlerod building, Guelph, Ontario, N1G 2W1, Canada

* To whom correspondence should be addressed.
Igor Kovalchuk, E-mail: igor.kovalchuk{at}uleth.ca


  Abstract

Here we analyzed the influence of salt stress on plant genome stability. Homologous recombination events were detected in transgenic Arabidopsis plants that carried in their genome {beta}-glucuronidase recombination marker. Recombination events were scored as sectors of blue using stereoscope. Exposure to 50 mM salt resulted in 3.0 fold increase in recombination frequency. To analyze the organ and tissue specificity of recombination events we performed cross sections of leaves, stems and roots. We found that nearly 30% of recombination events in plants grown at normal conditions and nearly 50% of events in plants grown on salt were undetected by conventional method.

Most of the recombination events represented a cluster/group of cells (12 on average); although, events with single cells were also detected. Recombination events were very frequent in leaf mesophyll cells. On average, individual recombination events located on leaves contained more cells than events located on roots or stems. Analysis of recombination events in crossectioned tissue of salt-treated plants revealed the shift in the distribution of recombination events towards the vascular tissue. We discuss the significance of finding for plant stress physiology.

Keywords: homologous recombination; tissue specific; vascular tissue; salt stress; Arabidopsis thaliana.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
H. Yin, X. Zhang, J. Liu, Y. Wang, J. He, T. Yang, X. Hong, Q. Yang, and Z. Gong
Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase {epsilon} Mutation in Arabidopsis
PLANT CELL, February 1, 2009; 21(2): 386 - 402.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.