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Plant and Cell Physiology Advance Access published online on March 29, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj040
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received February 4, 2006
Accepted March 18, 2006

Regular Paper

Expressed Sequence Tag Analysis of Lilium longiflorum Generative Cells

Takashi Okada 1, Prem L. Bhalla 2, and Mohan B. Singh 2 *

1 Plant Molecular Biology and Biotechnology laboratory, Australian Research Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria 3010, Australia; Present address: CSIRO Plant Industry, P.O. Box 350, Glen Osmond, SA 5064, Australia
2 Plant Molecular Biology and Biotechnology laboratory, Australian Research Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria 3010, Australia

* To whom correspondence should be addressed.
Mohan B. Singh, E-mail: mohan{at}unimelb.edu.au


  Abstract

The generative cell, male gametic cell progenitor in flowering plants undergoes mitotic division to produce two sperm cells. We have examined the gene-expression profile of the Lilium longiflorum (lily) generative cell by sequencing the expressed sequence tags (ESTs). A total of 886 ESTs derived from the generative-cell cDNA library were clustered into 637 unique ESTs comprising 123 cluster ESTs and 514 singleton ESTs. Thirty-nine percent of nonredundant ESTs showing similarity to Arabidopsis genes with known function were thus assigned putative functions. Genes related to the ubiquitin system were abundant, suggesting the key role of ubiquitin-dependent proteolysis in gametogenesis. Totals of 168 and 129 nonredundant lily generative-cell ESTs showed significant similarity to maize sperm-cell ESTs and Arabidopsis male gametophyte-specific transcripts, respectively. Fifty five ESTs appeared to have significant similarities to both maize sperm-cell ESTs and Arabidopsis male gametophyte-specific genes pointing towards conservation of male gamete expressed genes are conserved across different plant genera. Thus our data provides a handle to identify Arabidopsis gamete expressed genes and to investigate their function. Several of these genes are potential candidates for analyzing the molecular basis of fertilization and for investigating mechanisms of gamete-specific transcriptional regulation in Arabidopsis through bioinformatics-based approaches.


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