Plant and Cell Physiology Advance Access published online on March 13, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj034
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; The Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian 271018, China
* To whom correspondence should be addressed. Systemic defense response of tomato plant in response to insect attack and wounding is regulated by the 18-amino-acid peptide systemin and the phytohormone jasmonic acid (JA). Recent genetic analyses based mainly on the spr (suppressors of prosystemin-mediated responses) mutant screens have led to the hypothesis that systemin acts at, or near the site of wounding to amplify the production of JA, which in turn functions as a mobile signal to promote systemic defense response. In order to identify more components involved in the systemin/JA-signaled defense response, we carried out a larger scale screen for new spr mutants in tomato. Here we describe the characterization of spr6, a mutant impaired in wound- and systemin-induced defense gene expression. Using a candidate gene approach based on genetic linkage, we demonstrate that spr6 is allelic to jai1-1, which is a loss of function allele of the tomato homolog of CORONATIN-INSENSITIVE1 (COI1), an F-box protein that is required for JA-signaled processes in Arabidopsis. We show several aspects of the spr6 mutant phenotype is distinct from that of jai1-1. First, the responsiveness of spr6 plants to exogenous JA shows a dosage dependent manner, i.e., it is more sensitive to JA than jai1-1 while less sensitive to JA than wild-type. Second, unlike the sterile jai1-1, the spr6 plant displays normal fertility and seed set and thus, can be maintained as a pure line and does not require selection. Therefore, spr6 provides a valuable tool, which can complement the limitations of jai1-1, to study JA signaling in tomato. The gene identification process of Spr6 we described herein represents an example showing the convenience of a candidate gene approach, based on genetic linkage, to identify gene functions of genetic loci defined by tomato wound response mutants. 6 These authors contributed equally to this work.
Received December 25, 2005
Accepted March 9, 2006
Regular Paper
The Wound-Response Mutant suppressor of prosystemin-mediated responses6 (spr6) Is a Weak Allele of the Tomato Homolog of CORONATINE-INSENSITIVE1 (COI1)
Changbao Li 1 6,
Jiuhai Zhao 2 6,
Hongling Jiang 3,
Xiaoyan Wu 3,
Jiaqiang Sun 3,
Chunqing Zhang 4,
Xia Wang 5,
Yonggen Lou 5,
and
Chuanyou Li 3 *
2 State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Graduate School of Chinese Academy of Sciences, Beijing 100039, China
3 State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
4 The Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian 271018, China
5 Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China
Chuanyou Li, E-mail: cyli{at}genetics.ac.cn
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Shoji, T. Ogawa, and T. Hashimoto Jasmonate-Induced Nicotine Formation in Tobacco is Mediated by Tobacco COI1 and JAZ Genes Plant Cell Physiol., July 1, 2008; 49(7): 1003 - 1012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhai, C.-B. Li, W. Zheng, X. Wu, J. Zhao, G. Zhou, H. Jiang, J. Sun, Y. Lou, and C. Li Phytochrome Chromophore Deficiency Leads to Overproduction of Jasmonic Acid and Elevated Expression of Jasmonate-Responsive Genes in Arabidopsis Plant Cell Physiol., July 1, 2007; 48(7): 1061 - 1071. [Abstract] [Full Text] [PDF]
|
|