Skip Navigation



Plant and Cell Physiology Advance Access published online on March 9, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj030
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/5/634    most recent
pcj030v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lemaitre, T.
Right arrow Articles by Hodges, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemaitre, T.
Right arrow Articles by Hodges, M.
Agricola
Right arrow Articles by Lemaitre, T.
Right arrow Articles by Hodges, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received March 1, 2006
Accepted March 6, 2006

Regular Paper

Expression Analysis of Arabidopsis thaliana NAD-Dependent Isocitrate Dehydrogenase Genes Shows the Presence of a Functional Subunit that Is Mainly Expressed in the Pollen and Absent from Vegetative Organs

Thomas Lemaitre 1 and Michael Hodges 1 *

1 Institute de Biotechnologie des Plantes (CNRS UMR8618), Bâtiment 630, Université de Paris Sud-XI, 91405 Orsay cedex, France

* To whom correspondence should be addressed.
Michael Hodges, E-mail: hodges{at}ibp.u-psud.fr


  Abstract

NAD-dependent isocitrate dehydrogenase (IDH) is a Krebs cycle enzyme situated in mitochondria. In Arabidopsis thaliana, five genes encode functional IDH subunits that can be classed into two groups based on gene structure and subunit amino acid sequence. Arabidopsis contains two ‘catalytic’ and three ‘regulatory’ subunits according to their homology with yeast IDH. To date, an active IDH is believed to be heteromeric, containing at least one of each subunit type. This was verified in Arabidopsis by the complementation of yeast IDH mutants with the different Arabidopsis IDH encoding cDNAs. Indeed, a single ‘catalytic’ and ‘regulatory’ subunit was sufficient to restore acetate growth of the yeast IDH double mutant. To gain information on possible IDH subunit interactions in planta, Arabidopsis IDH gene expression was analysed by Northern blot, PCR on cDNA libraries, in silico and ‘promoter’-reporter gene transgenic plants. Four of the IDH genes were expressed in all plant organs tested while one gene (At4g35650) was not expressed in vegetative organs but mainly expressed in the pollen. In leaves, the IDH genes were highly expressed in the veins, and to a lesser extent in mesophyll cells. The data are discussed with respect to IDH in other plant species.

Keywords: Arabidopsis thaliana; gene expression; Krebs cycle; NAD-dependent isocitrate dehydrogenase; pollen; yeast mutant complementation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
C. P. Lee, H. Eubel, N. O'Toole, and A. H. Millar
Heterogeneity of the Mitochondrial Proteome for Photosynthetic and Non-photosynthetic Arabidopsis Metabolism
Mol. Cell. Proteomics, July 1, 2008; 7(7): 1297 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. G. Forde and P. J. Lea
Glutamate in plants: metabolism, regulation, and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2339 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Lemaitre, E. Urbanczyk-Wochniak, V. Flesch, E. Bismuth, A. R. Fernie, and M. Hodges
NAD-Dependent Isocitrate Dehydrogenase Mutants of Arabidopsis Suggest the Enzyme Is Not Limiting for Nitrogen Assimilation
Plant Physiology, July 1, 2007; 144(3): 1546 - 1558.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.