Plant and Cell Physiology Advance Access published online on February 18, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj022
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1 Department of Chemistry, Faculty of Science and Technology, Sophia University, Tokyo, Japan
* To whom correspondence should be addressed. The seismonastic movement of Mimosa pudica is triggered by a sudden loss of turgor pressure. In the present study, we compared the cell cytoskeleton by immunofluorescence analysis before and after movement, and the effects of actin- and microtubule-targeted drugs were examined by injecting them into the cut pulvinus. We found that fragmentation of actin filaments and microtubules occurs during bending, although the actin cytoskeleton, but not the microtubules, was involved in regulation of the movement. Transmission electron microscopy revealed that actin cables became loose after the bending. We injected phosphatase inhibitors into severed pulvinus to examine the effects of such inhibitors on the actin cytoskeleton. We found that changes in actin isoforms, fragmentation of actin filaments, and the bending movement were all inhibited after injection of a tyrosine phosphatase inhibitor. We thus propose that the phosphorylation status of actin at tyrosine residues affects dynamic reorganization of actin filaments and causes seismonastic movement.
Received July 22, 2005
Accepted February 15, 2006
Regular Paper
Change in the Actin Cytoskeleton During Seismonastic Movement of Mimosa pudica
Nobuyuki Kanzawa 1 *,
Yoshinori Hoshino 1,
Makiko Chiba 1,
Daisuke Hoshino 1,
Hidetaka Kobayashi 1,
Naomi Kamasawa 2,
Yoshiro Kishi 3,
Masako Osumi 2,
Masazumi Sameshima 4,
and
Takahide Tsuchiya 1
2 Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Japan
3 Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Tokyo, Japan
4 Department of Biofunctional Science, Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan
Nobuyuki Kanzawa, E-mail: n-kanza{at}sophia.ac.jp
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