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Plant and Cell Physiology Advance Access published online on February 1, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj017
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received December 29, 2005
Accepted January 26, 2006

Short Communication

Studies on Transcriptional Regulation of Endogenous Genes by ERF2 Transcription Factor in Tobacco Cells

Toshitsugu Nakano 1, Takumi Nishiuchi 2, Kaoru Suzuki 3, Tatsuhito Fujimura 4, and Hideaki Shinshi 3 *

1 Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennôdai, Tsukuba, Ibaraki 305-8572, Japan
2 Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 13-1 Takaramachi, Kanazawa 920-0934, Japan
3 Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
4 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennôdai, Tsukuba, Ibaraki 305-8572, Japan

* To whom correspondence should be addressed.
Hideaki Shinshi, E-mail: h.shinshi{at}aist.go.jp


  Abstract

In this study, we showed that overexpression of ethylene-responsive transcription factor (ERF) 2 activated the expression of endogenous genes that have the GCC box in their promoter region, in tobacco plants. These include not only a defense-related gene, CHN50, encoding class I basic chitinase, but also a transcriptional repressor gene, ERF3. In tobacco plants constitutively expressing ERF2:GR fusion protein, treatment with dexamethazone induced rapid increase of ERF3 mRNA and slow increase of CHN50 mRNA. These results suggest that an antagonistic interplay of ERF2 and ERF3 is involved in the transcriptional regulation of the class I basic chitinase genes in tobacco.

Keywords: chitinase; ERF2; ERF3; GCC box; tobacco; transcription.
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