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Plant and Cell Physiology Advance Access published online on January 24, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj014
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received December 16, 2005
Accepted January 19, 2006

Short Communication

An Alloplasmic Male-Sterile Line of Brassica oleracea Harboring the Mitochondria from Diplotaxis muralis Expresses a Novel Chimeric Open Reading Frame, orf72

Tomotaka Shinada 1 2, Yosuke Kikuchi 1 2, Ryo Fujimoto 1, and Sachie Kishitani 1 *

1 Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan

* To whom correspondence should be addressed.
Sachie Kishitani, E-mail: kishitan{at}bios.tohoku.ac.jp


  Abstract

Nuclear so-called fertility-restorer genes reverse the pollen sterility of cytoplasmic male sterile (CMS) plants caused by disturbed mitochondrial-nuclear interactions. We identified a CMS-associated chimeric mitochondrial gene in an alloplasmic CMS line of Brassica oleracea in the 'mur' system. This novel chimeric gene, orf72, was found in the mitochondrial genome of donor cytoplasm. It was located downstream of normal rps7 and contained part of atp9 (atp9-b). It was expressed specifically on the nuclear background of CMS B. oleracea, partially suppressed in the fertility-restored line and entirely suppressed in the cytoplasmic donor.

Keywords: Alloplasm; Brassica oleracea; cytoplasmic male sterility; Diplotaxis muralis; mitochondrial gene; orf72.

2The first two authors contributed equally to this work.


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