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Plant and Cell Physiology Advance Access published online on January 17, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pcj009
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Plant and Cell Physiology 2006 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received October 17, 2005
Accepted January 9, 2006

Regular Paper

Action Spectrum for Expression of the High Intensity Light-Inducible Lhc-like Gene Lhl4 in the Green Alga Chlamydomonas reinhardtii

Haruhiko Teramoto 1, Asako Ishii 2, Yukihiro Kimura 3, Koji Hasegawa 3, Shigeaki Nakazawa 2, Takanori Nakamura 4, Sho-ichi Higashi 4, Masakatsu Watanabe 5, and Taka-aki Ono 3 *

1 Laboratory for Photo-Biology (1), Photodynamics Research Center, RIKEN, Sendai 980-0845, Japan; Molecular Membranes Laboratory, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan; Present address: Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizu-cho Soraku-gun, Kyoto 619-0292, Japan
2 Laboratory for Photo-Biology (1), Photodynamics Research Center, RIKEN, Sendai 980-0845, Japan
3 Laboratory for Photo-Biology (1), Photodynamics Research Center, RIKEN, Sendai 980-0845, Japan; Molecular Membranes Laboratory, RIKEN, 2-1 Hirosawa, Saitama 351-0198, Japan
4 National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan
5 National Institute for Basic Biology, Myodaiji, Okazaki, Aichi 444-8585, Japan; Department of Photoscience, School of Advanced Science, Graduate University for Advanced Studies, Shonan Village, Hayama, Kanagawa 240-0193, Japan

* To whom correspondence should be addressed.
Taka-aki Ono, E-mail: takaaki{at}riken.jp


  Abstract

Lhl4 encodes a distant relative of light-harvesting chlorophyll-a/b proteins in the green alga Chlamydomonas reinhardtii. Lhl4 mRNA markedly accumulated within 30 min after illumination and in proportion to the light intensity up to a fluence rate much higher than that required for photosynthesis. The high-intensity light (HL)-induced accumulation of Lhl4 mRNA required continuous illumination, and the mRNA level rapidly decreased when the cells were placed in the dark. HL only slightly stabilized the mRNA, suggesting that the HL-induced expression of the Lhl4 gene is primarily regulated at the level of transcription. Blue light was more effective for inducing Lhl4 gene expression than green or red light, and far-red light had no effect. The action spectrum for Lhl4 gene expression was examined at the wavelength between 325 and 775 nm using the Okazaki Large Spectrograph. The obtained spectrum showed a distinct peak in the blue region (450 nm) and a shoulder in the UV-A region (375 nm). The curve in the spectrum rose steeply in the short-wavelength UV region. In addition, we observed two minor peaks in the green (575 nm) and the red (675 nm) regions. The action spectrum suggests that a blue/UV-A light photoreceptor with a flavin-based chromophore participates in the HL response of Lhl4 gene expression. However, the hypersensitivity to near UV-B light suggests the involvement of an unidentified UV-light perception system in the expression of the Lhl4 gene.

Keywords: action spectrum; blue/UV-light photoreceptor; Chlamydomonas reinhardtii; gene expression; high-light response; Lhc supergene family.
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