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Plant and Cell Physiology Advance Access published online on January 19, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci502
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights researved.
Received October 28, 2004
Accepted November 7, 2004

Mini Review

Molecular Genetics Using T-DNA in Rice

Gynheung An 1*, Shinyoung Lee 1, Sung Hyun Kim 2, and Seong-Ryong Kim 2

1 National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea
2 Department of Life Science, Sogang University, Seoul 121-742, Korea

* To whom correspondence should be addressed.
Gynheung An, E-mail: genean{at}postech.ac.kr


  Abstract

Now that sequencing of the rice genome is nearly completed, functional analysis of its large number of genes is the next challenge. Because rice is easy to transform, T-DNA has been successfully used to generate insertional mutant lines. Collectively, several laboratories throughout the world have established at least 200,000 T-DNA insertional lines. Some of those carry the GUS or GFP reporters for either gene or enhancer traps. Others are activation-tagging lines for gain-of-function mutagenesis when T-DNA is inserted in the intergenic region. Forward genetic approach showed limited success because of somaclonal variations induced during tissue culture. To utilize these resources more efficiently, tagged lines have been produced for reverse-genetics approaches. DNA pools of the T-DNA tagged lines have been prepared for PCR-screening of insertional mutants in a given gene. Appropriate T-DNA insertion sites are determined by sequencing the region flanking the T-DNA. This information is then used to make databases that are shared with the scientific community. International efforts on seed amplification and maintenance are needed to efficiently exploit these valuable materials.

Keywords: DNA pools; functional genomics; gene tagging; reverse genetics; Tag end sequence; T-DNA.
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