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Plant and Cell Physiology Advance Access published online on December 28, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci251
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received November 16, 2005
Accepted December 23, 2005

Short Communication

A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics

Hyeon-Jin Sun 1 2, Sayaka Uchii 1 2, Shin Watanabe 1, and Hiroshi Ezura 1 *

1 Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Ibaraki, Japan

* To whom correspondence should be addressed.
Hiroshi Ezura, E-mail: ezura{at}gene.tsukuba.ac.jp


  Abstract

We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1RifR harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.

Keywords: Agrobacterium tumefaciens (Rhizobium radiobacter); Functional genomics; Lycopersicon esculentum L.; Micro-Tom; Transformation.

2 These authors equally contributed to this study.


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