Skip Navigation



Plant and Cell Physiology Advance Access published online on January 17, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pci249
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/3/332    most recent
pci249v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Talbott, L. D.
Right arrow Articles by Zeiger, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Talbott, L. D.
Right arrow Articles by Zeiger, E.
Agricola
Right arrow Articles by Talbott, L. D.
Right arrow Articles by Zeiger, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received October 21, 2005
Accepted December 19, 2005

Regular Paper

Reversal by Green Light of Blue Light Stimulated Stomatal Opening in Intact, Attached Leaves of Arabidopsis Operates Only in the Potassium-Dependent, Morning Phase of Movement

Lawrence D. Talbott 1, Jamila W. Hammad 1, Lucy Cien Harn 1, Vi Hai Nguyen 1, Jaynita Patel 1, and Eduardo Zeiger 1 *

1 Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 900 Veteran Avenue, Los Angeles, CA 90024, USA

* To whom correspondence should be addressed.
Eduardo Zeiger, E-mail: zeiger{at}biology.ucla.edu


  Abstract

Green light reversal of blue light-stimulated, stomatal opening was discovered in isolated stomata. The present study shows that the response also occurs in stomata from intact leaves. Arabidopsis thaliana plants were grown in a growth chamber under blue, red and green light. Removal of the green light opened the stomata and restoration of green light closed them to baseline values under experimental conditions that rule out a mesophyll-mediated effect. Assessment of the response to green light over a daily time course showed that the stomatal sensitivity to green light was observed only in the morning, which coincided with the use of potassium as a guard cell osmoticum. Sensitivity to green light was absent during the afternoon phase of stomatal movement, which was previously shown to be dominated by sucrose osmoregulation in Vicia faba. Hence, the shift away from potassium-based osmoregulation in guard cells is further postulated to entail a shift from blue light to photosynthesis as the primary component of the stomatal response to light. Stomata from intact leaves of the zeaxanthin-less, npq1 mutant of arabidopsis, failed to respond to the removal or restoration of green light in the growth chamber, or to short, high fluence pulses of blue or green light. These data confirm previous studies showing that npq1 stomata are devoid of a specific blue light response. In contrast, stomata from intact leaves of phot1 phot2 double mutant plants had a reduced but readily detectable response to the removal of green light and to blue and green pulses.

Keywords: Arabidopsis; Blue light; Green light; Guard cells; Potassium; Zeaxanthin.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
K. M. Folta and S. A. Maruhnich
Green light: a signal to slow down or stop
J. Exp. Bot., September 1, 2007; 58(12): 3099 - 3111.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Banerjee, E. Schleicher, S. Meier, R. M. Viana, R. Pokorny, M. Ahmad, R. Bittl, and A. Batschauer
The Signaling State of Arabidopsis Cryptochrome 2 Contains Flavin Semiquinone
J. Biol. Chem., May 18, 2007; 282(20): 14916 - 14922.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.