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Plant and Cell Physiology Advance Access published online on January 11, 2006
Plant and Cell Physiology, doi:10.1093/pcp/pci248
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received June 28, 2005
Accepted December 15, 2005

Regular Paper

The Non-Host Pathogen Botrytis cinerea Enhances Glucose Transport in Pinus pinaster Suspension Cultured Cells

Herlânder Azevedo 1, Carlos Conde 1, Hernâni Gerós 1, and Rui Manuel Tavares 1 *

1 Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal

* To whom correspondence should be addressed.
Rui Manuel Tavares, E-mail: tavares{at}bio.uminho.pt


  Abstract

Botrytis cinerea is the causal agent of grey mould disease and a non-host necrotrophic pathogen of maritime pine (Pinus pinaster). Recent evidences suggest that pathogen challenging can alter carbon uptake in plant cells; however little is known on how elicitor-derived signalling pathways control sugar transport activity. P. pinaster suspended cells are able to absorb D-[14C]glucose by a high affinity, H+-dependent transport system (Km, 0.07 mM; Vmax, 1.5 nmol min-1 mg-1 DW), specific for D-glucose, D-fructose, D-galactose and D-xylose, and subject to glucose repression. When elicited by B. cinera spores, suspended cells exhibit calcium-dependent biphasic ROS production, being the second burst also dependent on NADPH oxidase, MAPK, and de novo transcription and protein synthesis. Challenging of suspended cells incubated in sugar-free medium resulted in an up to 3-fold increase in glucose transport capacity over non-elicited cultures 24 h after elicitation, and a 14-fold increase over elicited cells incubated with 2% glucose. Enhanced glucose uptake depended on NADPH oxidase and calcium influx, but not MAPK. In contrast, the increase of glucose transport activity induced by sugar starvation was dependent on the activation of MAPK but not NADPH oxidase. Both responses appeared to be dependent on de novo transcription and protein synthesis.

Keywords: Botrytis cinerea; Monosaccharide transporter; Non-host resistance; Oxidative burst; Pinus pinaster; Sugar uptake.
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