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Plant and Cell Physiology Advance Access published online on December 16, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci246
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received July 5, 2005
Accepted December 12, 2005

Short Communication

Cytosolic Dehydroascorbate Reductase Is Important for Ozone Tolerance in Arabidopsis thaliana

Seiji Yoshida 1 7, Masanori Tamaoki 2 * 7, Takeshi Shikano 3, Nobuyoshi Nakajima 2, Daisuke Ogawa 4, Motohide Ioki 2, Mitsuko Aono 5, Akihiro Kubo 5, Hiroshi Kamada 6, Yasunori Inoue 3, and Hikaru Saji 5

1 Master's Program in Biosystem Studies, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan; Biodiversity Conservation Research Project, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan
2 Biodiversity Conservation Research Project, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan
3 Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
4 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan; Biodiversity Conservation Research Project, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan
5 Environmental Biology Division, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan
6 Master's Program in Biosystem Studies, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan

* To whom correspondence should be addressed.
Masanori Tamaoki, E-mail: mtamaoki{at}nies.go.jp


  Abstract

Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDHAR. The mutant completely lacked cytDHAR activity and was highly ozone sensitive. The amounts of total ascorbate and glutathione were similar in both lines, but the amount of apoplastic ascorbate in the mutant was 61.5% lower. These results indicate that the apoplastic ascorbate, which is generated through the reduction of DHA by cytDHAR, is important for ozone tolerance.

Keywords: Arabidopsis thaliana; apoplast; ascorbate; dehydroascorbate reductase; oxidative stress; ozone.

7 These authors contributed equally to this work


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