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Plant and Cell Physiology Advance Access published online on December 16, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci243
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received October 13, 2005
Accepted December 8, 2005

Regular Paper

Accumulation of the Two Transcripts for the N Gene, Conferring Resistance to Tobacco Mosaic Virus, Is Likely Important for N Gene-Dependent Hypersensitive Cell Death

Reona Takabatake 1, Shigemi Seo 1, Ichiro Mitsuhara 1, Shinnya Tsuda 2, and Yuko Ohashi 1 *

1 National Institute of Agrobiological Sciences (NIAS), Kannon-dai, Tsukuba, Ibaraki, 305-8602 Japan
2 National Agricultural Research Center, Tsukuba Ibaraki 305-8666, Japan

* To whom correspondence should be addressed.
Yuko Ohashi, E-mail: yohashi{at}affrc.go.jp


  Abstract

The N gene is a Toll/interleukin-1 receptor (TIR)-nucleotide binding site (NBS)-leu-rich repeat (LRR)-type resistance (R) gene that generates two alternative transcripts, NS and NL. NS encodes the full-length N protein while NL is predicted to encode a truncated form of the protein (Ntr) lacking most of the LRR region. We found that the two transcripts were accumulated at 20°C, a permissive temperature, but not at 30°C, a non-permissive temperature for the N gene, in tobacco mosaic virus (TMV)-inoculated leaves. When N gene-dependent cell death was triggered by transient 20°C-treatment for 2 - 6 h, considerable levels of the transcripts were accumulated just before cell death, although the levels of NS were always higher. The accumulation was induced by transient expression of the 50 kDa helicase domain (p50) of TMV replicase which is the Avr component of N, but not by transient expression of NtMEK2DD-mediated cell death or N gene-independent hypersensitive cell death. These results suggest that the accumulation of NS and NL is associated with the function of N and above a certain threshold, triggers N-mediated hypersensitive cell death.

Keywords: Alternative-splicing; Hypersensitive cell death; N resistance gene; Temperature sensitive; Tobacco mosaic virus.
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