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Plant and Cell Physiology Advance Access published online on December 13, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci241
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received October 27, 2005
Accepted December 6, 2005

Regular Paper

Gibberellin Regulates Mitochondrial Pyruvate Dehydrogenase Activity in Rice

Asad Jan 1, Hidemitsu Nakamura 2, Hirokazu Handa 2, Hiroaki Ichikawa 2, Hiroshi Matsumoto 3, and Setsuko Komatsu 2 *

1 National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan; University of Tsukuba, Tsukuba 305-8572, Japan
2 National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
3 University of Tsukuba, Tsukuba 305-8572, Japan

* To whom correspondence should be addressed.
Setsuko Komatsu, E-mail: skomatsu{at}affrc.go.jp


  Abstract

Pyruvate dehydrogenase kinase (PDK) is a negative regulator of the mitochondrial pyruvate dehydrogenase complex (mtPDC) that plays a key role in intermediary metabolism. OsPDK1 was identified as a gibberellin (GA) up-regulated gene using a cDNA microarray. The full-length cDNA for OsPDK1 was 1498 bp and encoded a predicted polypeptide of 363 amino acids. Genomic DNA analysis showed the presence of another isoform of PDK, OsPDK2, in rice. Reverse transcriptase-PCR analysis revealed differential expression of the two isoforms. OsPDK1 was expressed in leaf blade and leaf sheath but not in callus and root, while OsPDK2 was expressed constitutively in all tissues examined. Maximum expression of OsPDK1 in leaf sheath was detected by Northern blot analysis when seedlings were treated with 5 µM GA3 for 24 h. OsPDK1 expression was up-regulated by GA3, and there was little effect of other plant hormones. Mitochondrial PDH activity was reduced compared to control plants in two-week-old seedlings treated with GA3. The GUS reporter gene, driven by a 2067bp OsPDK1 promoter region fragment, was mainly expressed in the aleurone layer of germinating seed and leaf sheath. Transgenic rice expressing PDK1 RNAi had altered vegetative growth with reduced accumulation of vegetative tissues. These results suggest that GA modulates the activity of mtPDC by regulating OsPDK1 expression and subsequently controlling plant growth and development.

Keywords: rice; leaf sheath; elongation; gibberellin; mitochondrial pyruvate dehydrogenase.
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